Gc ms solution software v 4

The oleoresin essential oils from L. formosana were subjected to gas chromatographic–mass spectral (GC–MS) analysis, as previously reported [34 (link)]: Shimadzu GCMS-QP2010 Ultra, electron impact (EI) mode with electron energy = 70 eV, scan range = 40–400 atomic mass units, scan rate = 3.0 scans/s, and Shimadzu GC-MS solution software v. 4.45 (Shimadzu Scientific Instruments, Columbia, MD, USA); ZB-5ms fused silica capillary GC column Phenomenex, Torrance, CA, USA; (5% phenyl)-polymethylsiloxane stationary phase, 0.25 μm film thickness; helium carrier gas, column head pressure = 552 kPa, flow rate = 1.37 mL/min; injector temperature = 260 °C, ion source temperature = 260 °C; GC oven temperature program: initial temperature = 50 °C, temperature increased 2 °C/min to 260 °C. For each sample, a 5% w/v solution in CH₂Cl₂ was prepared, and 0.1 μL was injected using a split ratio of 30:1. Identification of the individual components of the essential oils was determined by comparison of the Kovats retention indices, determined using a series of n-alkanes, in addition to comparison of the mass spectral fragmentation patterns with those found in the MS databases [35 ,36 ,37 ,38 ], using the LabSolutions GCMS solution software version 4.45 (Shimadzu Scientific Instruments, Columbia, MD, USA) and with matching factors >90%.