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Acer qpcr sybr green master mix

Manufactured by Vazyme
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Sourced in China

The AceR qPCR SYBR Green Master Mix is a ready-to-use solution for quantitative real-time PCR (qPCR) analysis. It contains SYBR Green I dye, hot-start DNA polymerase, and optimized buffer components for efficient and sensitive DNA amplification.

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Lab products found in correlation

Pcr master mix, by Vazyme (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

QPCR Master Mix SYBR qPCR Mix SYBR Master Mix SYBR Green Mix SYBR Green

2 protocols using acer qpcr sybr green master mix

1

MALAT1 Expression Quantification

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Total RNA was extracted from cells or mouse lung tissue using TRIzol reagent according to manufacturer’s instructions (Invitrogen, New York, United States). RNA was reverse-transcribed to complementary DNA (cDNA) using PCR Master Mix (Vazyme Biotech, Jiangsu, China). Real-time quantitative PCR was performed using an AceR qPCR SYBR Green Master Mix (Vazyme Biotech, Jiangsu, China). The expression of MALAT1 was normalized to GAPDH and calculated using the 2–ΔΔCt method. The primers used for qRT-PCR are listed in Table 3.
Gu H., Zhu Y., Zhou Y., Huang T., Zhang S., Zhao D, & Liu F. (2020). LncRNA MALAT1 Affects Mycoplasma pneumoniae Pneumonia via NF-κB Regulation. Frontiers in Cell and Developmental Biology, 8, 563693.
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2

RNA Extraction and qRT-PCR Analysis

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Total RNA was extracted from cells using Trizol reagent according to manufacturers' instructions (Ambion, Austin, USA). Then RNA was reverse translated to complementary DNA using the PCR Master Mix (Vazyme Biotech, Nanjing, China). qRT-PCR was performed using an AceR qPCR SYBR Green Master Mix (Vazyme Biotech) according to the manufacturer's instructions. The expression of NKILA was normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) RNA expression and calculated using the 2−ΔΔCt method. The primers used for qRT-PCR are listed in Table 3.
Zhang F., Zhang J., Liu F., Zhou Y., Guo Y., Duan Q., Zhu Y., Zhao D, & Gu H. (2021). Attenuated lncRNA NKILA Enhances the Secretory Function of Airway Epithelial Cells Stimulated by Mycoplasma pneumoniae via NF-κB. BioMed Research International, 2021, 6656298.
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