A total of 3×105 A549, H1299, or H1975 cells were seeded into 6-cm plates and incubated with different concentrations (0, 12.5, and 25 nM) of RocA for 24 h. The expression of CCL5 was analyzed by using a human CCL5 ELISA Kit (R&D Systems) according to the manufacturer's instructions. The plates were read using a spectrophotometer (BioTek) at an excitation wavelength of 450 nm and an emission wavelength of 570 nm.
Human ccl5 elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human CCL5 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure the concentration of human CCL5 (C-C Motif Chemokine Ligand 5) in cell culture supernatants, serum, and plasma. The kit utilizes a pair of antibodies specific for human CCL5 to capture and detect the analyte. A series of standards with known concentrations of human CCL5 are used to generate a standard curve, which allows the determination of the unknown sample concentrations.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using human ccl5 elisa kit
1
Analyzing CCL5 Expression in Cancer Cells
2
Coculture Cytokine Profiling of OSCC Cells and Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
YD-38 cells cocultured with fibroblasts in 24-well transwell plates were treated with or without rhBMP-2 (1 ng/ml) for 48 h, and the supernatants were then subjected to the human cytokine antibody array (Ray Biotech, GA, USA). The relative expression level of cytokines was determined by comparing signal intensities.
Monoculture supernatants were obtained by culturing OSCC cell lines or fibroblasts on the same side of filters as described in the coculture design. Cells were cultured for 48 h with either 1 ng/ml rhBMP-2-containing medium or pure serum-free medium. The CCL5 levels in supernatants obtained from monoculture or coculture models were quantified using the Human CCL5 ELISA Kit (R&D Systems, MN, USA). Experiments were performed three times under identical conditions.
Monoculture supernatants were obtained by culturing OSCC cell lines or fibroblasts on the same side of filters as described in the coculture design. Cells were cultured for 48 h with either 1 ng/ml rhBMP-2-containing medium or pure serum-free medium. The CCL5 levels in supernatants obtained from monoculture or coculture models were quantified using the Human CCL5 ELISA Kit (R&D Systems, MN, USA). Experiments were performed three times under identical conditions.
3
PDAC Cells Conditioned Media CCL5 Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PDAC cell lines (1 × 107cells) were cultured for 72 h and the conditioned media collected after centrifugation at 700 g for 5 min at 4 °C. CCL5 protein was quantified using human CCL5 ELISA kit (R&D Systems) according to the manufacturer's instructions.
4
Profiling Chemokine Secretion in Glioma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The experimental procedure followed the manufacturer's instructions for the human chemokine antibody array C1 (AAH-CHE-1; RayBiotech, Inc., Norcross, GA). Conditioned media acquired from U87 cells and U87 GSCs were applied to the membranes for 2 h. After washing, array antibodies and horseradish peroxidase-conjugated streptavidin were incubated with the membranes for 2 h. The membranes were then added to 1Â detection buffers, and photographs were obtained using a charge-coupled device camera. The reagent of the human CCL5 ELISA kit was used (R&D Systems, Minneapolis, MN), and the expression test was performed strictly according to the instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!