(R)-ketamine hydrochloride was prepared by recrystallization of (R,S)-ketamine (Ketalar®, ketamine hydrochloride, Daiichi Sankyo Pharmaceutical Ltd., Tokyo, Japan) and D-(−)-tartaric acid, as described previously25 (link). The purity of (R)-ketamine was determined by a high-performance liquid chromatography (CHIRALPAK® IA, Column size: 250 × 4.6 mm, Mobile phase: n-hexane/dichloromethane/diethylamine (75/25/0.1), Daicel Corporation, Tokyo, Japan). The dose (10 mg/kg as ketamine hydrochloride) of (R)-ketamine and lanicemine (AZD6765; Sigma-Aldrich Co. Ltd, St Louis, MO, USA) was used as previously reported26 (link)–30 (link),57 (link).
Azd6765
Manufactured by Merck Group
Sourced in United States
AZD6765 is a laboratory instrument designed for scientific research. It is a product of Merck Group, a leading global pharmaceutical and life science company. The core function of AZD6765 is to facilitate analytical and experimental procedures within a controlled laboratory environment. No additional details are available for this product at this time.
Automatically generated - may contain errors
Lab products found in correlation
Chiralpak ia, by Daicel (1 mentions)
N hexane dichloromethane diethylamine 75 25 0.1, by Daicel (1 mentions)
Rapamycin, by Cayman Chemical (1 mentions)
Cp 101 606, by Merck Group (1 mentions)
Bafilomycin a1 baf a1, by Abcam (1 mentions)
Dmem f12, by Welgene (1 mentions)
L glutamic acid, by Merck Group (1 mentions)
2 protocols using azd6765
1
Synthesis and Characterization of (R)-Ketamine
2
ARPE-19 Cell Culturing and Compound Treatments
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human RPE cell line, ARPE-19, was cultured in Dulbecco’s modified Eagle medium, F-12 (DMEM F-12; WELGENE, Gyeongsan, Korea) containing 10% fetal bovine serum (FBS) at 37 °C in a humidified incubator with 5% CO2. Ro 25-6981 maleate salt, L-glutamic acid, AZD6765, and CP-101,606 were purchased from Sigma-Aldrich (St. Louis, MI, USA). A2E was purchased from Key Synthesis LLC (Philadelphia, PA, USA). Rapamycin was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA). Bafilomycin A1 (Baf A1) was purchased from Abcam (Cambridge, UK).
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