Lung mononuclear cells (MNCs) were re-suspended in FACS buffer (cat. no. 00-4222; eBioscience, Inc.) (2×106 cells/ml) and stained with a PE-cluster of differentiation (CD) 11c antibody (cat. no. 561044; eBioscience; Thermo Fisher Scientific, Inc.) and APC-Cy7-F4/80 antibody (cat. no. 123117; BioLegend, Inc.) to characterize the alveolar macrophages (AMs) as CD11c and F4/80 double-positive cells. The antibodies fluorescein isothiocyanate (FITC)-major histocompatibility complex (MHC) II (cat. no. 556643), FITC-CD80 (cat. no. 553768), FITC-CD40 (cat. no. 561845), FITC-CD206 (cat. no. 551135), FITC-IL-10 (cat. no. 554466; eBioscience Inc.) and APC-CD86 (cat. no. 102513; BioLegend, Inc.) were used to perform the second cell surface staining. All cells were analyzed by flow cytometry (FACSAria III; BD Bioscences). The results were analyzed using FlowJo (version 10.0.7r2; FlowJo LLC)
Apc cy7 f4 80 antibody
Manufactured by BioLegend
Sourced in United States
The APC-Cy7-F4/80 antibody is a fluorescently labeled antibody that binds to the F4/80 antigen, which is expressed on the surface of macrophages and related cells. This antibody can be used for the identification and analysis of macrophage populations in flow cytometry applications.
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Lab products found in correlation
Cd86 apc, by BioLegend (2 mentions)
Facsaria 3, by BD (2 mentions)
B220 fitc, by Thermo Fisher Scientific (1 mentions)
Mhcii fitc, by Thermo Fisher Scientific (1 mentions)
Cd40 fitc, by Thermo Fisher Scientific (1 mentions)
Cd80 fitc, by Thermo Fisher Scientific (1 mentions)
Cd11b apc, by BioLegend (1 mentions)
2 protocols using apc cy7 f4 80 antibody
1
Characterizing Alveolar Macrophages by Flow Cytometry
2
Characterization of Lung and Bone Marrow-Derived Dendritic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To determine the phenotype of DCs in lung tissue and BMDCs, prepared lung mononuclear cells (MNCs) and BMDCs were suspended in a FACS buffer at 2 × 106 cells/ml. DCs express the marker CD11c but not the marker F4/80 on the cell surface; therefore, DCs were gated as CD11c+/F4/80− cells using the PE-CD11c antibody (eBioscience, San Diego, CA, USA) and the APC-Cy7-F4/80 antibody (BioLegend Inc., San Diego, CA, USA). Next, the cells were stained with the following antibodies: FITC-MHCII, FITC-CD80, FITC-CD40, FITC-B220, FITCICAM-1 (eBioscience, San Diego, CA, USA), APC-CD86, APC-CCR7, APC-CD11b (Biolegend Inc., San Diego, CA, USA). All cells were analyzed by flow cytometry (FACSAria™ III, BD Biosciences, USA).
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