RAW264.7, HeLa, 3T3, HePG2, MG-63, PC3, 293T and A549 cell lines were purchased from the Cell Bank of the Chinese Academy of Science Type Culture Collection (China). Cells were cultured according to the supplier’s instructions.
LPS from Escherichia coli O55:B5 (Sigma-Aldrich, USA; # L-2637) and recombinant human tumor necrosis factor-alpha (TNF-ɑ) (Sangon Biotech, China; # C600021) were used to induce inflammation. Progesterone, aldosterone, PMA, Azd9567 and Dex were purchased from MCE (Medchem Express, USA), and and all the tested compounds were bought from Chemdiv and the compounds are listed in Supplementary TableS1 . pGL4.36[luc2P MMTV Hygro] (GenBank accession no. FJ773214) was purchased from Promega. pCMV-GR11 (Addgene, USA; # 89105), pCMV-hAR (Addgene, USA; # 89078), and pcDNA3-PRB (Addgene, USA; # 89130) were gifts from Elizabeth Wilson. pNF-κB-luc (Beyotime Biotechnology, China; #D2206) was purchased from Beyotime. pCMV-hMR was constructed by cloning MR fragment into the plasmid of pCMV-hAR by the technology of seamless cloning (Vazyme, China; # C112-1). AP-1-luc was constructed by cloning five copies of AP-1 promoter into the BmtI and BglII sites of pNF-κB-luc. ARR3tk promoter was cloned into the HindIII and XhoI sites of PGL4.18 vector (GenBank accession no. DQ188838) (Promega, USA).
LPS from Escherichia coli O55:B5 (Sigma-Aldrich, USA; # L-2637) and recombinant human tumor necrosis factor-alpha (TNF-ɑ) (Sangon Biotech, China; # C600021) were used to induce inflammation. Progesterone, aldosterone, PMA, Azd9567 and Dex were purchased from MCE (Medchem Express, USA), and and all the tested compounds were bought from Chemdiv and the compounds are listed in Supplementary Table