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Monocyte colony stimulating factor

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Monocyte-colony stimulating factor is a protein that promotes the differentiation and proliferation of monocytes and macrophages, which are important components of the immune system. It is a key regulator of myeloid cell production and function.

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Lab products found in correlation

Collagenase type 4, by Merck Group (2 mentions) Anti pe conjugated ferromagnetic beads, by Miltenyi Biotec (2 mentions)

Close spelling variants of this product

Granulocyte monocyte colony stimulating factor (2 citations)

2 protocols using monocyte colony stimulating factor

1

Isolation and Culture of Murine Intestinal Macrophages

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Murine intestinal macrophages were isolated by immunomagnetic cells separation. Briefly, intestinal tissue was digested with HBSS containing 1 mM collagenase type IV (Sigma) × 2 h at 37°C. Macrophages were first stained with phycoerythrin (PE)-conjugated anti-Ly6C and anti-F4/80 antibodies and then separated using anti-PE-conjugated ferromagnetic beads (Miltenyi Biotec, San Diego, CA). More than 90% of these cells were confirmed as CD11b+ F4/80+ CD11cint macrophages by FACS.
BMDMs were prepared (37 (link)) by culturing bone marrow cells in DMEM with 10% FCS and 40 ng/mL monocyte-colony stimulating factor (PeproTech, Rocky Hill, NJ). After 5 days, BMDMs were collected by scraping and cultured in RPMI with 10% FCS.
MohanKumar K., Namachivayam K., Chapalamadugu K., Garzon S.A., Premkumar M.H., Tipparaju S, & Maheshwari A. (2016). Smad7 Interrupts TGF-β Signaling in Intestinal Macrophages and Promotes Inflammatory Activation of these Cells during Necrotizing Enterocolitis. Pediatric research, 79(6), 951-961.
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2

Isolation and Culture of Murine Intestinal Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine intestinal macrophages were isolated by immunomagnetic cells separation. Briefly, intestinal tissue was digested with HBSS containing 1 mM collagenase type IV (Sigma) × 2 h at 37°C. Macrophages were first stained with phycoerythrin (PE)-conjugated anti-Ly6C and anti-F4/80 antibodies and then separated using anti-PE-conjugated ferromagnetic beads (Miltenyi Biotec, San Diego, CA). More than 90% of these cells were confirmed as CD11b+ F4/80+ CD11cint macrophages by FACS.
BMDMs were prepared (37 (link)) by culturing bone marrow cells in DMEM with 10% FCS and 40 ng/mL monocyte-colony stimulating factor (PeproTech, Rocky Hill, NJ). After 5 days, BMDMs were collected by scraping and cultured in RPMI with 10% FCS.
MohanKumar K., Namachivayam K., Chapalamadugu K., Garzon S.A., Premkumar M.H., Tipparaju S, & Maheshwari A. (2016). Smad7 Interrupts TGF-β Signaling in Intestinal Macrophages and Promotes Inflammatory Activation of these Cells during Necrotizing Enterocolitis. Pediatric research, 79(6), 951-961.
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