Cytometric bead array flex set system

Manufactured by BD

Sourced in United States

The Cytometric Bead Array Flex Set system is a multiplex assay platform that enables the simultaneous quantification of multiple soluble analytes in a single sample. It utilizes flow cytometry technology to detect and measure the concentration of targeted proteins or other biomolecules.

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Lab products found in correlation

Lsrii fortessa analyzer, by BD (1 mentions) Fcap array software version 3, by BD (1 mentions) Facscalibur apparatus, by BD (1 mentions) Cytokine elisa kit, by BD (1 mentions) Luminex instrument, by Merck Group (1 mentions) Ebio64dec17, by Thermo Fisher Scientific (1 mentions) Milliplex nhp kits, by Merck Group (1 mentions) Anti ifn γ 4s b3, by BioLegend (1 mentions) Golgistop, by BD (1 mentions) Fcap array software, by BD (1 mentions)

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Cytometric bead array (430 citations) Flex Sets (25 citations)

8 protocols using cytometric bead array flex set system

Cytokine Profiling of T-cell Culture

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Culture supernatants as prepared above (T-cell division) were collected after 3, 72, and 144 h of culture to measure the production of human IL-2, interferon-γ (IFN-γ), tumor necrosis factor (TNF), GM-CSF, and Granzyme B using BD Cytometric Bead Array Flex Set System (BD Bioscience).

Iriguchi S., Yasui Y., Kawai Y., Arima S., Kunitomo M., Sato T., Ueda T., Minagawa A., Mishima Y., Yanagawa N., Baba Y., Miyake Y., Nakayama K., Takiguchi M., Shinohara T., Nakatsura T., Yasukawa M., Kassai Y., Hayashi A, & Kaneko S. (2021). A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy. Nature Communications, 12, 430.

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Cytokine Profiling in Sepsis

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Cytokines (interleukin-6, keratinocyte chemoattractant, monocyte chemoattractant protein-1, tumor necrosis factor-α, and interleukin-10) in peritoneal lavage supernatant, serum collected at 24 h after cecal ligation and puncture, and peritoneal macrophage media supernatant were evaluated with the BD Cytometric Bead Array Flex Set System (BD ACCURI C6, BD Biosciences) according to the manufacturer's instructions.

Kusakabe Y., Uchida K., Yamamura Y., Hiruma T., Totsu T., Tamai Y., Tsuyuzaki H., Hasegawa K., Chang K, & Yamada Y. (2018). Early-phase Innate Immune Suppression in Murine Severe Sepsis Is Restored with Systemic Interferon-β. Anesthesiology, 129(1).

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Cytokine Profiling using Bead Array

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Serum or plasma cytokine levels were assessed using a murine soluble protein Cytometric Bead Array Flex Set System (BD Biosciences), as per manufacturer’s instructions. Data were acquired on a BD LSR II Fortessa Analyzer (BD Biosciences) and analyzed using FCAP Array Software version 3.0 (BD Biosciences).

Lansink L.I., Skinner O.P., Engel J.A., Lee H.J., Soon M.S., Williams C.G., SheelaNair A., Pernold C.P., Laohamonthonkul P., Akter J., Stoll T., Hill M.M., Talman A.M., Russell A., Lawniczak M., Jia X., Chua B., Anderson D., Creek D.J., Davenport M.P., Khoury D.S, & Haque A. (2023). Systemic host inflammation induces stage-specific transcriptomic modification and slower maturation in malaria parasites. mBio, 14(4), e01129-23.

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Cytokine Measurement in RAW264 Cells

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Cytokines in the culture supernatant were measured with a cytometric bead array flex set system (BD Biosciences, San Jose, CA, USA), according to the manufacturer’s protocol. Briefly, RAW264 cells in 24-well plates were exposed to 10 µg/mL FT9110 for 24 hours, and cytokine capture beads (for TNF, RANTES, MIP-1α, MCP-1, IL-1β, IL-10, and IL-6) were added to the samples or cytokine standards (10–2,500 pg/mL) in flow cytometry tubes. The mixtures were vortexed, and antibodies for fluorescence detection were added to each tube. The samples were then incubated at room temperature for 2 hours. Following incubation, the beads were washed once and resuspended prior to reading with a FACSCalibur apparatus (BD Biosciences). Test media were assayed in triplicate for each treatment condition.

Kuroda C., Ueda K., Haniu H., Ishida H., Okano S., Takizawa T., Sobajima A., Kamanaka T., Yoshida K., Okamoto M., Tsukahara T., Matsuda Y., Aoki K., Kato H, & Saito N. (2018). Different aggregation and shape characteristics of carbon materials affect biological responses in RAW264 cells. International Journal of Nanomedicine, 13, 6079-6088.

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Quantification of Metabolic Markers and Cytokines

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Glucose, lactate, and triacylglycerol were determined using commercially available enzymatic assay kits (BioAssay Systems or BioVision) following the manufacturers' instructions. Glycerol 3-phosphate and activity of cG3PDH were quantified as previously described (58 (link), 59 (link)). Cytokines were measured using the Cytometric Bead Array Flex Set system (BD Biosciences) and analyzed with FlowJo (Tree Star, Inc.). Cytokine detection limits are IL-1β: 1.9 pg/mL; IL-6: 1.4 pg/mL; IL-10: 9.6 pg/mL; and TNF-α: 2.8 pg/mL.

Rosas-Ballina M., Guan X.L., Schmidt A, & Bumann D. (2020). Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis. Frontiers in Immunology, 11, 131.

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Cytokine and Chemokine Profiling

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Cytokine ELISA kits were used to quantify IFN-γ, IL-12p70 and TNF-α (BD Biosciences and Biolegend). Cytokine and chemokine bead arrays (LINCOplex) were used to quantify IFN-γ, IL-6, -10, -13, TNF-α, GM-CSF, CXCL8, CXCL10, CCL3 and CCL4 and samples analysed on a Luminex instrument (all Millipore). IL-6, -10, GM-CSF, CCL5, CXCL10 and CCL2 were also measured using a Cytometric Bead Array Flex Set system (BD Biosciences).

Robson N.C., Hidalgo L., Mc Alpine T., Wei H., Martínez V.G., Entrena A., Melen G.J., MacDonald A.S., Phythian-Adams A., Sacedón R., Maraskovsky E., Cebon J., Ramírez M., Vicente A, & Varas A. (2014). Optimal effector functions in human natural killer cells rely upon autocrine bone morphogenetic protein signaling. Cancer research, 74(18), 5019-5031.

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Cytokine Profiling of Activated T Cells

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For intracellular cytokine staining, T cells were activated for 3h with PMA and ionomycin in the presence of GolgiStop (BD Bioscience), followed by anti-IFNγ (4S.B3; Biolegend) or anti-IL-17 (eBio64Dec17; ebiosciences). Levels of IFNγ and IL-17 secreted by T cells stimulated for 4 days under Th1-, Th2- or Th17-polarizing conditions were measured using a Cytometric Bead Array (CBA) Flex Set system (BD Bioscience) and analyzed with FCAP Array Software (BD Bioscience). Cytokine levels in serum were determined by the University of Pittsburgh Cancer Institute Luminex Core Laboratory using MILLIPLEX NHP kits from Millipore (Billerica, MA), according to the manufacturer’s instructions.

Ezzelarab M.B., Zhang H., Guo H., Lu L., Zahorchak A.F., Wiseman R.W., Nalesnik M.A., Bhama J.K., Cooper D.K, & Thomson A.W. (2016). Regulatory T Cell Infusion Can Enhance Memory T Cell and Alloantibody Responses in Lymphodepleted Nonhuman Primate Heart Allograft Recipients. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(7), 1999-2015.

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Cytokine Quantification in Choroid Plexus

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IL‐6, IL‐8, and CCL2 were assayed in supernatants collected from choroid plexus fibroblast/leukaemia cell co‐cultures using commercially available ELISA kits (BioLegend) or the Cytometric Bead Array (CBA) Flex Set system (BD Biosciences), following the manufacturer's recommendations.

Fernández‐Sevilla L.M., Valencia J., Flores‐Villalobos M.A., Gonzalez‐Murillo Á., Sacedón R., Jiménez E., Ramírez M., Varas A, & Vicente Á. (2020). The choroid plexus stroma constitutes a sanctuary for paediatric B‐cell precursor acute lymphoblastic leukaemia in the central nervous system. The Journal of Pathology, 252(2), 189-200.

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