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Pge2 elisa

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The PGE2 ELISA is a laboratory assay used to quantify the levels of Prostaglandin E2 (PGE2) in biological samples. It employs the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure the concentration of PGE2 present in the sample.

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Lab products found in correlation

Pgd2 mox elisa, by Cayman Chemical (1 mentions) Mouse xl cytokine array kit, by R&D Systems (1 mentions)

Close spelling variants of this product

ELISA kits of PGE2 PGE2 ELISA kit PGE2 ELISA assay kit ELISA kits for PGE2 PGE2 ELISA Kit (Mouse PGE2, Mouse PGE2 ELISA kit Enzyme-linked immunosorbent assay (ELISA) kits for PGE2 ELISA) kit for PGE2 Enzyme-linked immunosorbent assay (ELISA) kit for PGE2 ELISAs

5 protocols using pge2 elisa

1

Quantifying Secreted PGE2 and IL1β

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PGE2 in the supernatant of cultured cells, mouse serum, and human BM samples was determined by PGE2 ELISA (Cat#KGE004B), and human IL1β in mouse serum and human BM samples was determined by ELISA specific for human IL1β (Cat#DLB50) (both from R&D Systems; Minneapolis, MN) following the manufacturer’s instructions.
Carter B.Z., Mak P.Y., Wang X., Tao W., Ruvolo V., Mak D., Mu H., Burks J.K, & Andreeff M. (2019). An ARC-regulated IL1β/Cox-2/PGE2/β-catenin/ARC circuit controls leukemia-microenvironment interactions and confers drug resistance in AML. Cancer research, 79(6), 1165-1177.
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2

Quantifying Secreted PGE2 in Cell Culture

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For measuring secreted PGE2, conditioned media was collected after 24 hours of treatment and a PGE2 ELISA (R&D Systems) was run according to the manufacturer’s specifications.
Kastrati I., Litosh V.A., Zhao S., Alvarez M., Thatcher G.R, & Frasor J. (2015). A novel aspirin prodrug inhibits NFκB activity and breast cancer stem cell properties. BMC Cancer, 15, 845.
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3

Cytokine and Prostaglandin Profiling

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CM was collected from each group after centrifugation at 700 × g for 10 min at 4°C and stored at −80°C for subsequent experiments. For cytokine measurement, conditioned medium was subjected to a proteome profiler array using a mouse XL cytokine array kit (R&D, ARY028), and the relative levels of 111 cytokines and chemokines were measured. The array procedure and data analysis were performed according to the manufacturer’s instructions.
For measurement of prostaglandins, CM were collected as described and analyzed according to the manufacturer’s instructions. The ELISA kit used for measurement were as follows: PGD2-MOX ELISA(Cayman Chemical company, 500151); PGJ2 ELISA(ENZO, ADI-900-023); PGE2ELISA(R&D systems, KGE004B).
Lemaire V., Koehl M., Le Moal M, & Abrous D.N. (2000). Prenatal stress produces learning deficits associated with an inhibition of neurogenesis in the hippocampus. Proceedings of the National Academy of Sciences of the United States of America, 97(20), 11032-11037.
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4

Radiation-induced PGE2 production in primary cells

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Primary cells were prepared as described above. On day 2 of culture, media was replaced and cells were treated with indomethacin (25 μM in saline) or vehicle (saline with 0.1% ethanol) 1 h prior to receiving a 5 Gy dose of radiation. Cell-free supernatants were collected at indicated timepoints following radiation and the PGE2 concentrations were determined with an enzyme-linked immunosorbent assay (PGE2 ELISA, R&D Systems, no. KGE004B, Minneapolis, MN) following the manufacturer’s instructions, where PGE2 was normalized to the protein concentration of the corresponding cell culture dish.
Gilman K.E., Camden J.M., Woods L.T., Weisman G.A, & Limesand K.H. (2021). Indomethacin Treatment Post-irradiation Improves Mouse Parotid Salivary Gland Function via Modulation of Prostaglandin E2 Signaling. Frontiers in Bioengineering and Biotechnology, 9, 697671.
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5

Quantifying PGE2 Levels in Cell Culture

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Prostaglandin E2 (PGE2) ELISA (R&D Systems, Minneapolis, MN, USA) was performed on medium collected 24 h post-impact. The collected medium was centrifuged at 12,000× g at room temperature to separate out any debris prior to performing the assay per the manufacturer’s instructions.
Karnik S., Noori-Dokht H., Williams T., Joukar A., Trippel S.B., Sankar U, & Wagner D.R. (2023). Decreased SIRT1 Activity Is Involved in the Acute Injury Response of Chondrocytes to Ex Vivo Injurious Mechanical Overload. International Journal of Molecular Sciences, 24(7), 6521.
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