Lung digests were filtered through 100-μm strainers (Fisher), subjected to red-cell lysis (eBioscience), and stained with PE-labeled antimouse podoplanin (eBioscience) and APC-labeled antimouse LYVE-1 (R&D), with Aqua (Biolegend) viability marker for dead-cell exclusion. Dual PE/APC+ live cells were analyzed by an LSRII (BD) cytometer. The quantity of dual-positive cells as a percentage of total cells was analyzed/plotted and used in statistical analyses comparing lungs from mutant versus control mice.
Pe labeled antimouse podoplanin
Manufactured by Thermo Fisher Scientific
PE-labeled antimouse podoplanin is a fluorescently-labeled antibody that specifically binds to the podoplanin protein found on the surface of certain cell types. It can be used to detect and analyze podoplanin-expressing cells in various research applications.
Automatically generated - may contain errors
2 protocols using pe labeled antimouse podoplanin
1
Quantification of Podoplanin and LYVE-1 Expressing Cells
2
Quantitative Flow Cytometry Analysis of Lung Cells
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