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Prk5 myc rhoa t19n

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PRK5-myc-RhoA-T19N is a plasmid construct that expresses a mutant form of the RhoA protein. RhoA is a small GTPase that regulates various cellular processes. The T19N mutation in RhoA results in a dominant-negative form that can inhibit the activity of wild-type RhoA.

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Lab products found in correlation

Plentiekar2g2, by Addgene (1 mentions) Gw1 phred, by Addgene (1 mentions) Ptriex rhoa flare, by Addgene (1 mentions) Egfp p65, by Addgene (1 mentions) Pmitotimer, by Addgene (1 mentions) Mcherry lifeact 7, by Addgene (1 mentions) Pgp cmv gcamp6f, by Addgene (1 mentions) Pcmv flag yap2 5sa, by Addgene (1 mentions) 8xgtiic luciferase, by Addgene (1 mentions)

5 protocols using prk5 myc rhoa t19n

1

Murine Moloney Leukemia Virus Retroviral Vectors

Cited 2 times
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The murine Moloney leukemia virus-based retroviral vectors CAG–GFP [27 ], CAG-GFP/Cre [12 (link)], CAG-RFP [28 (link)], and CAG-GFP-T2A-CreERT2 [29 (link)] were kind gifts from Dr. Fred Gage and the retroviral CAG-IRES-DsRed vector [30 (link)] from Dr Benedikt Berninger. Rnd2 and DNRhoA were cloned by PCR using pNeuroD1-Rnd2 [22 (link)] and pRK5-myc-RhoA-T19N (Addgene 12963) as templates respectively and then inserted into the SfiI/PmeI sites of the CAG-Neurog2-IRES-DsRed retroviral vector [30 (link)] to generate CAG-Rnd2-IRES-DsRed and CAG-DNRhoA-IRES-DsRed.
Kerloch T., Farrugia F., Bouit L., Maître M., Terral G., Koehl M., Mortessagne P., Heng J.I., Blanchard M., Doat H., Leste-Lasserre T., Goron A., Gonzales D., Perrais D., Guillemot F., Abrous D.N, & Pacary E. (2021). The atypical Rho GTPase Rnd2 is critical for dentate granule neuron development and anxiety-like behavior during adult but not neonatal neurogenesis. Molecular Psychiatry, 26(12), 7280-7295.
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2

Comprehensive RhoA Signaling Toolkit

Cited 1 time
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Raichu-RhoA (provided by M. Matsuda [27 (link)]), pTriEx-RhoA FLARE.sc Biosensor WT (RRID:Addgene_12150), pTriEx-RhoA FLARE.sc Biosensor Q63L (RRID:Addgene_12151), pTriEx-RhoA FLARE.sc Biosensor T19N (RRID:Addgene_12152), pRK5-myc-RhoA WT (RRID:Addgene_12962), pRK5-myc-RhoA Q63L (RRID:Addgene_12964), pRK5-myc-RhoA T19N (RRID:Addgene_12963), EGFP-p65 (RRID:Addgene_111190), GW1-pHRed (RRID:Addgene_31473), GW1CMV-Perceval (RRID:Addgene_21737), Laconic/pcDNA3.1 (+) (RRID:Addgene_118627), Pyronic /pcDNA3.1 (+) (RRID:Addgene_51308), pcDNA3.1 FLII12Pglu-700uDelta6 (RRID:Addgene_17866), Cyto-ABKAR (RRID:Addgene_61510), pLentiEKAR2G2 (RRID:Addgene_40178), Kras-Src FRET biosensor (RRID:Addgene_78302), pFRET-HSP33 cys (RRID:Addgene_16076), pGP-CMV-GCaMP6F (RRID:Addgene_40755), mCherry-Lifeact-7 (RRID:Addgene_54491), pMitoTimer (RRID:Addgene_52659), pCLBW cox8 EGFP mCherry (RRID:Addgene_78520).
Socodato R., Rodrigues-Santos A., Tedim-Moreira J., Almeida T.O., Canedo T., Portugal C.C, & Relvas J.B. (2023). RhoA balances microglial reactivity and survival during neuroinflammation. Cell Death & Disease, 14(10), 690.
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3

Moloney Leukemia Virus-Based Retroviral Vectors

Cited 2 times
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The murine Moloney leukemia virus-based retroviral vectors CAG–GFP 27 (link)
, CAG-GFP/Cre 12 (link)
, CAG-RFP 28 (link)
 and CAG-GFP-T2A-CreERT229 (link)
 were kind gifts from Dr Fred Gage and the retroviral CAG-IRES-DsRed vector 30 (link)
 from Dr Benedikt Berninger. Rnd2 and DNRhoA were cloned by PCR using pNeuroD1-Rnd2 22 (link)
 and pRK5-myc-RhoA-T19N (Addgene 12963) as templates respectively and then inserted into the SfiI/PmeI sites of the CAG-Neurog2-IRES-DsRed retroviral vector 30 (link)
 to generate CAG-Rnd2-IRES-DsRed and CAG-DNRhoA-IRES-DsRed.
Kerloch T., Farrugia F., Bouit L., Maître M., Terral G., Koehl M., Mortessagne P., Heng J.I., Blanchard M., Doat H., Leste-Lasserre T., Goron A., Gonzales D., Perrais D., Guillemot F., Abrous D.N, & Pacary E. (2021). The atypical Rho GTPase Rnd2 is critical for dentate granule neuron development and anxiety-like behavior during adult but not neonatal neurogenesis. Molecular Psychiatry, 26(12), 7280-7295.
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4

Dominant Negative GTPase Regulation

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Dominant negative forms of Rho family GTPase were purchased from Addgene as gifts of Dr. Gary Bokoch (Scripps Institute): pRK5-myc-RhoA-T19N (RhoA-DN, #12963), pRK5-myc-Rac1-T17N (Rac1-DN, #12984), and pRK5-myc-Cdc42-T17N (Cdc42-DN, #129730). IFN-luc was kindly provided by Dr. Wang-shik Ryu (Yonsei University, Korea). 8XGTIIC-luciferase (containing YAP/TAZ-responsive synthetic promoter; Addgene #34615) was a gift from Dr. Stefano Piccolo (University of Padova, Italy). pCMV-flag-YAP2-5SA (Constitutive active form of YAP; Addgene #27371) was a gift from Dr. Kunliang Guan (University of California San Diego, CA).
Kim J., Kang W., Kang S.H., Park S.H., Kim J.Y., Yang S., Ha S.Y, & Paik Y.H. (2020). Proline-rich tyrosine kinase 2 mediates transforming growth factor-beta-induced hepatic stellate cell activation and liver fibrosis. Scientific Reports, 10, 21018.
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5

Murine Retroviral Vectors for Gene Delivery

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The murine Moloney leukemia virus-based retroviral vectors CAG-GFP (Zhao et al., 2006) , CAG-GFP/Cre (Tashiro et al., 2006a) , CAG-RFP (Laplagne et al., 2006) and CAG-GFP-T2A-CreER T2 (Mu et al., 2015) were kind gifts from Dr Fred Gage and the retroviral CAG-IRES-DsRed vector (Heinrich et al., 2011) from Dr Benedikt Berninger. Rnd2 and DNRhoA were cloned by PCR using pNeuroD1-Rnd2 (Heng et al., 2008 ) and pRK5-myc-RhoA-T19N (Addgene 12963) as templates respectively and then inserted into the SfiI/PmeI sites of the CAG-Neurog2-IRES-DsRed retroviral vector (Heinrich et al., 2011) to generate CAG-Rnd2-IRES-DsRed and CAG-DNRhoA-IRES-DsRed.
Kerloch T., Farrugia F., Maître M., Terral G., Koehl M., Heng J.I., Blanchard M., Doat H., Lesté-Lasserre T., Goron A., Gonzales D., Guillemot F., Abrous D.N., & Pacary E. (2020). The atypical Rho GTPase Rnd2 is critical for dentate granule neuron development and anxiety-like behavior during adult but not neonatal neurogenesis.
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