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3 protocols using poly ethylene terephthalate pet

1

PET and Nylon 6 Fabric Enzymatic Treatment

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Poly(ethylene terephthalate) (PET) and polyamide 6 (nylon 6) fabrics, at thickness of 0.41 ± 0.015 mm and weight of 240 ± 12 g(m2)−1 were purchased from Goodfellow (Huntingdon, UK). All the other chemicals and solvents were purchased from Sigma-Aldrich (Vienna, Austria) at reagent grade and used without any further purification if not specified. The Humicola insolens cutinase (HiC) (CAS 9000-62-1) was purchased by Novozymes (Copenhagen, Denmark), and used without purification steps.
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2

Fabrication of Microfluidic Devices

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Chemicals and materials were obtained from the following sources and used without further purification: 2'-Deoxyadenosine-5'-monophosphate (Alfa Aesar); Thymidine-5'-monophosphate disodium salt (Alfa Aesar); 2'-Deoxycytidine-5'-monophosphate (Alfa Aesar); 2'-Deoxyguanosine-5'-monophosphate disodium salt hydrate (Alfa Aesar); potassium chloride (1 and 3 m, Sigma-Aldrich); Tris-EDTA (100×, Sigma-Aldrich); Tris-Borate-EDTA (10×, Sigma-Aldrich); silver (Ag) wires (>99%, Sigma-Aldrich); S1813 photoresist (MicroChem); MF319 developer (MicroChem); potassium hydroxide (KOH) pellets (Fisher Scientific); hydrofluoric acid (HF) (Sigma-Aldrich); Fluorolink MD 700 (Solvay); 2-hydroxy-2-methylpropiophenone (Sigma-Aldrich); NOA72 (Norland Products); poly(ethylene glycol) diacrylate (PEGDA) (Mn = 250, Sigma-Aldrich); 2,2-dimethoxy-2-phenylacetophenone (Sigma-Aldrich); Si wafers (P/B, resistivity 5-10 Ωcm, orientation of (100), and 525 ± 25 μm thickness) (WaferPro); polyethylene terephthalate (PET) with 250 μm thickness (Goodfellow); polymethylmethacrylate (PMMA) with a thickness of 250 μm (Goodfellow); polymethylmethacrylate (PMMA) of 1 mm thickness (ePlastics).
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3

Polymer Functionalization with NaSS

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Sodium 4-styrene sulfonate (NaSS), phosphate buffer saline (PBS), and Mohr's salt were purchased from Sigma Aldrich. Polyethylene terephthalate (PET) (0.25 mm, biaxially oriented plate) was purchased from Goodfellow (Paris, France). Ethanol absolute was from Fisher.
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