The PCR system for KASP genotyping consisted 2× Master Mix 96/384, High Rox, (LGC Genomics, Middlesex, UK). PCR were performed in an Applied Biosystems® Veriti® 384-Well Thermal Cycler. The fluorescent signals of PCR products were detected by BMG Omega F (LGC Genomics, UK), and Kluster Caller software was used to perform the statistics.
Veriti 384 well thermal cycler
The Veriti® 384-Well Thermal Cycler is a laboratory instrument used for DNA amplification by the polymerase chain reaction (PCR) process. It has a 384-well sample block that allows for simultaneous processing of multiple DNA samples. The Veriti® Thermal Cycler precisely controls the temperature and duration of the thermal cycling steps required for PCR, enabling reliable and reproducible DNA amplification.
Lab products found in correlation
3 protocols using veriti 384 well thermal cycler
KASP Genotyping of HvAnt1 and HvAnt2
The PCR system for KASP genotyping consisted 2× Master Mix 96/384, High Rox, (LGC Genomics, Middlesex, UK). PCR were performed in an Applied Biosystems® Veriti® 384-Well Thermal Cycler. The fluorescent signals of PCR products were detected by BMG Omega F (LGC Genomics, UK), and Kluster Caller software was used to perform the statistics.
Microsatellite Genotyping of Plant Samples
PCR fragments were run on an ABI3730 genetic analyzer (Applied Biosystems). Alleles were scored manually using Peak Scanner 1.0 (Applied Biosystems) and binned according to their motif length with Flexibin [25] .
Genotyping of JLH Chicken SNPs
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