Antibodies and their sources are listed: Notch3 and HIF‐1α (Abcam), smooth muscle actin (Sigma‐Aldrich), RBPjk (Santa Cruz), Hey L (Life Span Biosciences). Ki67 (Dako), anti‐acetylated tubulin and Quinomycin A (Sigma‐Aldrich), Dolichos biflorus agglutinin (DBA), and Lotus tetragonolobus agglutinin (LTA) (Vector Laboratories, Inc).
Hey l
Manufactured by LifeSpan BioSciences
Hey L is a versatile and high-performance laboratory equipment designed for a wide range of applications. It features advanced technology that enables precise and efficient sample processing. The core function of Hey L is to provide researchers and scientists with a reliable tool for their laboratory needs.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hey l
1
Comprehensive Immunohistochemical Characterization
2
Murine Model of Polycystic Kidney Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies and reagents: Antibodies and their sources are listed: Notch3 and HIF-1α from Abcam (Cambridge, MA), smooth muscle actin from (Sigma Aldrich, Saint Louis, MO), RBPjk (Santa Cruz, Dallas, TX), Hey L (Life Span Biosciences, Seattle, WA). Ki67 from Dako, Quinomycin A and anti-acetylated a tubulin from Sigma Aldrich (Saint Louis, MO). Dolichus biflorus agglutinin (DBA) was obtained from Vector Labs.
Animal care and protocol: Pkd1 RC/RC : Pkd2 +/-(PKD) mice were generated by breeding Pkd1 RC/RC mice with Pkd2 +/-mice (both in C57BL/6J background) kindly provided by Drs. Peter Harris and Steven Somlo, respectively (15, 16) . Homozygous Pkd1 RC/RC and heterozygous Pkd2 +/-mice have a slow progressing cyst phenotype. However, when these mice are bred to obtain PKD1 RC/RC , PKD2 +/-mice, cyst formation is accelerated. The PKD1 RC/RC , PKD2 +/-(PKD) mice are mildly cystic at birth and the cysts progress with age such that there is an exponential growth of the cysts between age P15 and P60 after which cyst growth is minimal (unpublished). We used these mice because the time period of cyst formation is moderate for the drug studies.
Wildtype littermates without a PKD mutation (Pkd1 +/+/ /Pkd2 +/+ ) were used as controls. Mice were generated at the PKD rodent core facility at the University of Kansas Medical Center.
Animal care and protocol: Pkd1 RC/RC : Pkd2 +/-(PKD) mice were generated by breeding Pkd1 RC/RC mice with Pkd2 +/-mice (both in C57BL/6J background) kindly provided by Drs. Peter Harris and Steven Somlo, respectively (15, 16) . Homozygous Pkd1 RC/RC and heterozygous Pkd2 +/-mice have a slow progressing cyst phenotype. However, when these mice are bred to obtain PKD1 RC/RC , PKD2 +/-mice, cyst formation is accelerated. The PKD1 RC/RC , PKD2 +/-(PKD) mice are mildly cystic at birth and the cysts progress with age such that there is an exponential growth of the cysts between age P15 and P60 after which cyst growth is minimal (unpublished). We used these mice because the time period of cyst formation is moderate for the drug studies.
Wildtype littermates without a PKD mutation (Pkd1 +/+/ /Pkd2 +/+ ) were used as controls. Mice were generated at the PKD rodent core facility at the University of Kansas Medical Center.
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