Poly (vinyl alcohol) (98% hydrolysis degree, Mw 13,000–23,000 g/mol) and methanol came from Sigma-Aldrich®, St. Louis, MO, USA. Poly(lactic acid) (Mw 200,000 g/mol) was a donation from Natureworks, Minnetonka, MN, USA. Dimethylsulfoxide, glutaraldehyde (aqueous solution 25% (v/v)), sodium chloride (Pro Analyses), dibasic sodium phosphate (P.A.) and chloroform were purchased from Vetec® Química Fina, Brazil. Potassium chloride (P.A.) was procured from Isofar, Brazil. Monobasic potassium phosphate (P.A.) was from Reagen, Brazil. Anforicin B (the amphotericin micellar system) was from Cristália (Itapira, São Paulo, Brazil), and amphotericin B drug powder (AmB) was purchased from Indofine Chemical Company, Inc. (Hillsborough, NJ, USA).
Glutaraldehyde
Manufactured by Vetec
Sourced in Brazil
Glutaraldehyde is a chemical compound used as a fixative and disinfectant in various laboratory applications. It is a colorless, oily liquid with a pungent odor. Glutaraldehyde is commonly used to preserve and fix biological samples for microscopy and other analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Sodium phosphate dibasic, by Vetec (1 mentions)
Methanol, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Vetec (1 mentions)
Sodium chloride (nacl), by Vetec (1 mentions)
Polylactic acid, by NatureWorks (1 mentions)
Chloroform, by Vetec (1 mentions)
Sodium nitroprusside, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Avantor (1 mentions)
Milli q plus, by Merck Group (1 mentions)
Acetic acid, by Vetec (1 mentions)
Knife mill, by Tecnal (1 mentions)
4 protocols using glutaraldehyde
1
Poly(vinyl alcohol)-based Hydrogel Formulation
2
Rat Reproductive Organ Morphometry
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At 90 days, all male rats were heparinized (125 IU/100g of body weight) and after 15 minutes, anesthetized intramuscularly with a combination of ketamine (50 mg/kg) and xylazine (5 mg/kg). Subsequently, a blood sample was taken from vena cava sinus and after clotting and centrifugation; the recovered serum was stored at -20 °C until further analysis for testosterone and prolactin measurement. An intracardiac perfusion was performed using a 0.9% NaCl solution plus heparin (500 IU l-1) and sodium nitroprusside (100 mg l-1; Sigma) for 5-10 min. After that, all rats were perfused using 4% glutaraldehyde (Vetec) in sodium phosphate buffer (0.01 M, pH 7.2) for 15 min. After fixation, the testes, epididymis, seminal gland and prostate were removed and weighed. The weighing of these androgen-dependent organs was done using a BEL Engineering scale (Mark 500/BRA) with 0.001 g precision.
3
Analytical Reagents for Scientific Research
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Apocynin (Acetovanillone 98%) and bovine serum albumin (BSA) (≥98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethyl alcohol was obtained from FMaia (São Francisco, MG, Brazil). Glutaraldehyde (25%) and acetic acid were from Vetec (Rio de Janeiro, RJ, Brazil). HPLC-grade acetonitrile was from JTBaker (Philippsburg, NJ, USA). For all analyses, ultrapure water was obtained from Millipore Milli-Q Plus apparatus (Millipore, Rio de Janeiro, RJ, Brazil). All solvents and chemicals used were of analytical or HPLC grade.
4
Synthesis of Pectin-PVA-Amylase Blends
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The synthesis of pectin/PVA/amylase blends was performed by varying the concentration of the cross-linking agent glutaraldehyde. Briefly, 2.4 g of lobeira pectin and 0.24 g of PVA were added to 20 mL of distilled water (Vetec Química Fina Ltda.). The solutions were kept stirred at room temperature (25°C), and, after homogenization, 2 mL of α-amylase (as in Section 2.1.1 ) was added. Thereafter, different volumes of 25% (v/v) glutaraldehyde (Vetec Química Fina Ltda.) were added to final concentrations of 0.25, 0.5, 0.75, 1.0, and 1.25% (v/v). The system was maintained with stirring for 10 min at 25°C. The resulting solutions were deposited on molds, and films were produced by casting. After being removed from the molds, the films were milled in a knife mill (Tecnal, Brazil) to obtain grids with an average size of 500 μm.
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