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Anti human ige clone g7 18

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Anti-human IgE (clone G7–18) is a monoclonal antibody that specifically binds to the Fc region of human immunoglobulin E (IgE) molecules. This antibody is used as a research tool for the detection and quantification of human IgE in various applications, such as immunoassays and flow cytometry.

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Lab products found in correlation

Europium conjugated streptavidin, by PerkinElmer (1 mentions) Legend max elisa kit, by BioLegend (1 mentions) Human ige full length protein, by Abcam (1 mentions)

Close spelling variants of this product

Anti-human IgE Clone 18; Anti-IgE

2 protocols using anti human ige clone g7 18

1

Quantification of Peanut-Specific IgE

Cited 1 time
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PN-specific IgE was quantified by ELISA, capturing IgE onto plates coated with 3µg/ml anti-human IgE (clone G7–18, BD Biosciences), and detecting with biotinylated PN extract (200ng/ml, see reference11 (link)). A standard curve was constructed using PN-allergic patient sera that had been previously quantified by Immunocap. Tryptase measurements were performed by ELISA as previously described12 (link).
Burton O.T., Stranks A.J., Tamayo J.M., Koleoglou K.J., Schwartz L.B, & Oettgen H.C. (2016). A humanized mouse model of anaphylactic peanut allergy. The Journal of allergy and clinical immunology, 139(1), 314-322.e9.
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2

Quantification of Cytokines and Immunoglobulins

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Cell culture supernatants were assayed for human or murine IFNγ, IL-4, IL-17A/F and murine GM-CSF using LEGEND MAX™ ELISA kits (BioLegend). Human plasma IgE was captured using anti-human IgE (clone G7-18, BD Biosciences). Human IgE full-length protein (Abcam) was the standard and biotinylated anti-human IgE (clone G7-26, BD Biosciences) and Europium-conjugated streptavidin (Perkin Elmer) were used for detection. Murine plasma IgE was captured using anti-mouse IgE (clone R35-72), with purified mouse IgE as the standard and biotinylated anti-mouse IgE (clone R35-118) for detection (BD Biosciences).
Dendrou C.A., Cortes A., Shipman L., Evans H.G., Attfield K.E., Jostins L., Barber T., Kaur G., Kuttikkatte S.B., Leach O.A., Desel C., Faergeman S.L., Cheeseman J., Neville M.J., Sawcer S., Compston A., Johnson A.R., Everett C., Bell J.I., Karpe F., Ultsch M., Eigenbrot C., McVean G, & Fugger L. (2016). Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity. Science translational medicine, 8(363), 363ra149.
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