Endocrine resistant LY2 breast cancer cells were a kind gift from Robert Clarke (Georgetown, USA). LY2 cells were derived from MCF-7 cells by selection in increasing concentrations of LY 117018 [53 (link)]. LY2 cells are also cross-resistant to tamoxifen and are ER positive with ER protein expression lower than MCF-7 cells [16 (link), 39 (link), 53 (link)]. LY2 cells were maintained in phenol red free Minimum Essential Medium Eagle (PRF-MEM, Sigma, Darmstadt, Germany) supplemented with 10% charcoal dextran stripped fetal calf serum (CDS-FCS, Sigma), 1% L-glutamine (LG, Sigma), 1% penicillin-streptomycin (PS, Sigma) and 4-hydroxytamoxifen (4-OHT, Sigma) 10−8 mol/L. A stable SRC-1 knock-down cell line was created, as previously described [39 (link)]. LY2 shSRC-1 and LY2 shNT cells were maintained in LY2 cell culture media containing puromycin (500 ng/mL, Sigma) to maintain stable knockdown expression. All cell lines were tested for Mycoplasma (LT07-118, Lonza, Basel, Switzerland), genotyped (Source BioSciences, Nottingham, UK) and authenticated according to ATCC guidelines.
L glutamine lg
Manufactured by Merck Group
Sourced in Germany, Denmark
L-glutamine (LG) is a naturally occurring amino acid that plays a critical role in cellular metabolism. It serves as a key substrate for various metabolic processes and is essential for maintaining cellular function and integrity. As a laboratory product, LG is used as a versatile component in cell culture media and other research applications.
Automatically generated - may contain errors
Lab products found in correlation
Heracell 150i, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin p s, by Merck Group (1 mentions)
Lt07 118, by Lonza (1 mentions)
4 hydroxytamoxifen 4 oht, by Merck Group (1 mentions)
Puromycin, by Merck Group (1 mentions)
Trypan blue, by Merck Group (1 mentions)
Type a, by Merck Group (1 mentions)
Streptomycin p s, by Lonza (1 mentions)
Trypsin, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Penicillin, by Lonza (1 mentions)
2 protocols using l glutamine lg
1
Characterization of Endocrine-Resistant LY2 Breast Cancer Cells
2
Murine C2C12 Myoblast Expansion Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Murine C2C12 SkM myoblasts (Blau et al., 1985 (link); Yaffe & Saxel, 1977 (link)) were first seeded (1 × 106 cells) onto pre‐gelatinized (0.2% in dH2O; Type A; Sigma‐Aldrich) T75 flasks (Nunc™; Thermo Fisher Scientific) within a Class II biological safety cabinet (BSC; Kojair) and expanded in growth media composed of high glucose (4.5 g/L) Dulbecco's modified Eagle's medium, including 4 mM l ‐Glutamine (LG; Sigma‐Aldrich), 10% heat‐inactivated fetal bovine serum (hiFBS; SLS), 10% heat‐inactivated newborn calf serum (hiNBCS; Fisher Scientific, Denmark), supplemented with an additional 2 mM LG (Lonza), 100 U/ml penicillin‐100 μg/ml streptomycin (PS; Lonza) in a humidified incubator (HERAcell 150i; Thermo Scientific) at 37°C, 5% CO2 until 80% confluency was attained. Once confluent, cells were washed twice with sterile phosphate‐buffered saline (1× PBS; Sigma‐Aldrich), Trypsinized (0.05% Trypsin/0.02% EDT; Sigma‐Aldrich), and counted using the trypan blue exclusion method (0.4% trypan blue; Sigma‐Aldrich).
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