Crystallinity and crystallographic orientation of 3NA nanocrystals inside the electrospun fibers was studied by X-ray diffraction. The diffraction pattern using θ–2θ scans was recorded between 10°and 60° on a Philips PW-1710 X-ray diffractometer with Cu-Kα radiation of wavelength 1.5406 Å. The lattice planes parallel to the substrate surface were determined from the reciprocal lattice vector of modulus (2/λ)sin θ, with λ the radiation wavelength and θ the Bragg angle. Raman spectroscopy was carried out on a LabRAM HR Evolution confocal Raman spectrometer (Horiba Scientific, France) using Horiba Scientific's Labspec 6 Spectroscopy Suite Software for instrument control, data acquisition and processing. The Raman spectra was obtained using a laser excitation with wavelength 532 nm, at 0.1% laser intensity, with 30 s acquisition time in a spectral range between 50–1750 cm−1.
Labspec 6 spectroscopy suite software
Manufactured by Horiba
Sourced in France
The Labspec 6 Spectroscopy Suite Software is a comprehensive software package designed for the analysis and interpretation of spectroscopic data. It provides a user-friendly interface for the acquisition, processing, and visualization of spectroscopic measurements.
Automatically generated - may contain errors
4 protocols using labspec 6 spectroscopy suite software
1
Crystallinity Analysis of 3NA Nanocrystals
2
Micro-Raman Analysis of Mineral Phases
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A LabRam HR800 micro-Raman from Horiba Scientific, equipped with an air-cooled CCD detector at − 70 °C, an Olympus BXFM microscope (objective 10 × and 50 ×), and a 600 groove/mm grating, was used to collect the Raman scattering signals of mineral phases present on the samples. The excitation source was a He–Ne laser (632.8 nm line) with a maximum laser power of 17 mW and the spectrometer was calibrated with silicon at 520 cm−1. Spectra acquisition and treatment were performed using HORIBA Scientific’s LabSpec 6 Spectroscopy Suite Software. Identification of the mineral phases and peaks attribution was done referring to BIO-RAD spectral database, using KnowItAll spectroscopy software.
3
Raman Spectroscopic Characterization of CNT, Lipid, and CNT-Lipid Samples
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CNT, lipid and CNT-lipid samples were dehydrated using nitrogen gas followed by vacuum drying in the desiccator for about 20 minutes. Spectroscopic measurements were carried out on a Horiba Jobin-Yvon LabRAM HR800 spectrometer. A 532 nm laser was utilized to collect spectra from 100-4000 cm -1 using a grating of 600 g mm -1 and blazed at 750 nm. Spectra were acquired using ×50 long working distance objective with a numerical aperture of 0.50. The confocal hole was set at 100 μm for spectral collections. The detector used was an Andor electromagnet (EM) charged coupled device (CCD). A video camera with the Raman system was used to guide spectral collection. All spectra were collected with sample situation on Calcium Fluoride slides (Crystran, UK). The instrumentation was calibrated before operation to silicon at the spectral line of 520.8 cm -1 . Spectra were acquired using the 532 nm laser at 1% exposure for 10 seconds and accumulated 5 times. Immediate data interrogation and manipulation was carried out on the raw data using the LabSpec 6 spectroscopy software suite (HORIBA Scientific).
4
SERS Detection of Pymetrozine using M-AgNPs
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Prior to Raman measurement, a portion of 200.0 μL M-AgNPs was mixed with 200.0 μL of pymetrozine solution in a 1.5 mL centrifuge tube, and the mixed solution was stirred for 8 s, then 40.0 μL of NaNO3 solution (1 mol/L) was added to the tube and mixed for 8 s to facilitate AgNPs aggregation. The mixed solution was analyzed after these preparations. For SERS detection, the above solution was first sucked into a capillary with an inner diameter of 1 mm and then analyzed with a laser confocal microscopic Raman system (LabRAM HR, Horiba France SAS, Villeneuve, France) equipped with a high stable confocal microscope (BX41, Olympus Co., Center Valley, PA, USA), a 633 laser radiation, a grating of 600 grooves/mm, and a cooled CCD with 1024 × 256 pixels’ sensor. All Raman spectra were collected in the range of 300–2000 cm−1, and the acquisition time was 30 s with 2 accumulations. Each sample were repeated three times, and the mean value was used for analysis.
The Raman spectra were acquired and analyzed using a LabSpec 6 spectroscopy software suite (Horiba France SAS, Villeneuve, France). Two spectral preprocessing methods including denoising and baseline correction were performed to improve the signal to noise ratio and to minimize the interference of fluorescence.
The Raman spectra were acquired and analyzed using a LabSpec 6 spectroscopy software suite (Horiba France SAS, Villeneuve, France). Two spectral preprocessing methods including denoising and baseline correction were performed to improve the signal to noise ratio and to minimize the interference of fluorescence.
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