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Acetone is a clear, colorless, and volatile liquid chemical compound. It has the chemical formula CH3COCH3 and is a common organic solvent used in various industrial and laboratory applications. Acetone is known for its ability to dissolve a wide range of materials, including plastics, paints, and adhesives.

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6 protocols using acetone

1

Assembling QCM Resonator Sensor

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N-Octadecyltrichlorosilane
(OTS) (95%) was purchased from Acros. Acetone was purchased from Shanghai
Chemical Reagent Co., Ltd. (Shanghai, China). QCM resonators with
silver electrode were purchased from Chengdu West Sensor Co., Ltd.,
China, and were used as substrates. The fundamental frequency of these
QCM resonators is 107 Hz (AT-cut). QCM resonators were
rinsed with ethanol for 10 min and dried with N2 before
being coated with PODS.
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2

Thiol-Mediated Photoredox Polymerization for Advanced Materials

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1-Butanethiol (Energy Chemical, Shanghai, China, 98%), 2-bromopropionic acid (Energy chemical, Shanghai, China, 99%), tetrabutylammonium hydrogen sulfate (Aladdin, Shanghai, China, 99%), 2,2’-(thiocarbonyldithio)diacetic acid, N,N-dimethyl acrylamide (DMA) (Aldrich, St. Louis, MO, USA, 99%), N,N-dimethylaminoethyl acrylate (DMAEA) (Energy chemical, Shanghai, China, 98%), N,N-Diethylacrylamide (DEA) (Aladdin, Shanghai, China, 99%), N-(3-(dimethylamino)propyl)acrylamide (DPAA) (Energy Chemical, Shanghai, China, 98%), 3-mercaptopropionic acid (Aladdin, Shanghai, China, 99%), 2-Bromoisobutyric acid (Energy chemical, Shanghai, China, 98%), 4,7-Di(thiophen-2-yl)benzo[c][1,2,5]thiadiazole (DTBT as photoredox catalyst in this study) (ARK, Chicago, IL, USA, >98.0%), bis(carboxymethyl) trithiocarbonate, CAS: 6326-83-6) (BCMT) (Energy Chemical, Shanghai, China, 98%), N, N-dimethylformamide (DMF) (Aladdin, Shanghai, China, 99.8%) were purchased from chemical company, respectively. Acetone, hydrochloric acid, carbon disulfide, chloroform, DCM, hexane, ether, K3PO4·3H2O, NaCl, NaOH, D2O, CDCl3, anhydrous Na2SO4 were obtained from Shanghai Chemical Reagent Co., Shanghai, China.
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3

Synthesis of Metal-Organic Frameworks

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All of the reagents were commercially available and used without any purification. Zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99.0%), zirconium tetrachloride (ZrCl4, 99.5%) and magnesium nitrate hexahydrate (Mg(NO3)2·6H2O, 99.0%) were obtained from Alfa Aesar. Dimethylformamide (DMF, 99.0%), methanol (CH3OH, 99.9%), acetone (CH3COCH3, 99.5%), ethanol (C2H5OH, 99.8%) and glacial acetic acid (CH3COOH, 99.99%) was purchased from Shanghai Chemical Reagent Co., Ltd (No. 52, Ningbo Road, Shanghai, China). 2,5-Dihydroxyterephthalic acid (99.0%), 2,5-dimethylterephthalic acid (99.0%) and 2-methylimidazole (99.0%) were obtained from Maclin. Carbon dioxide (CO2, 99.99%) was purchased from Shanghai Shenkai Co., Ltd. (No. 1769, Puxing Road, Shanghai, China).
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4

Fabrication of Thin-Film Electrodes

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Al and CuO sputter targets (7 cm in diameter, 6 mm in thickness) were purchased from Jiangxi Ketai Advanced Material Co. Ltd. Acetone and ethanol were bought from Shanghai Chemical reagent Co. Ltd, positive photoresist (BP212) was gained from Kempur Microelectronics Inc. The electrode plugs were fabricated industrially. Deionized water was used in all experiments in the study.
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5

Vasculature Protection Protocol

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TCE, Freund's complete adjuvant (FCA) and 4-Hydroxy-TEMPO (Tempol) were bought from Sigma (St. Louis, MO, USA); olive oil and acetone were purchased from the Shanghai Chemical Reagent Company (Shanghai, China); anti-rabbit monoclonal VCAM-1 antibody, antimouse monoclonal ICAM-1 antibody and anti-rabbit polyclonal CD62E (E-selectin) antibody were purchased from Abcam (Cambridge, UK); rabbit anti-mouse polyclonal eNOS antibody was bought from Bioss (Beijing, China); goat anti-rabbit secondary antibody, rabbit anti-mouse secondary antibody and phenylmethylsulfonyl fluoride (PMSF) were bought from Cell Signaling Technology (Danvers, MA, USA); radio immuno-precipitation lysis buffer (RIPA) and BCA Kit was from Beyotime (Shanghai, China); Creatinine Assay Kit, Urea Assay Kit, Nitric Oxide (NO) assay kit (Nitrate reductase method), AdeNOSinedeaminase assay kit, Superoxide Dismutase (SOD) assay kit (WST-1 method) and Malondialdehyde (MDA) assay kit (TBA method) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Immunohistochemistry kits, Histostain Plus and DAB substrate kits were purchased from ZSJQ-BIO (Beijing, China).
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6

Podocyte Injury Evaluation Protocol

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TCE, Freund's complete adjuvant (FCA) and Dimethyl sulfoxide (DMSO) were purchased from Sigma (St. Louis, MO, USA). Olive oil and acetone were from Shanghai Chemical Reagent Company (Shanghai, China). Cystatin C (Cys-C) and Podocalyxin (PCX) ELISA kits were from Elabscience Biotechnology Co., Ltd. (Wuhan, China). CTSL inhibitor, CTSL and Nephrin primary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-C3, anti-β actin primary antibodies and corresponding secondary antibodies used for IF were bought from Abcam (Cambridge, UK). IHC kit was from ZSGB Biotechnology Co., Ltd. (Beijing, China). Collagenase A was from Roche (Mannheim, Germany). Dynabeads M-450 Tosylactivated and magnetic particle concentrator were bought from ThermoFisher Scientific (Waltham, MA, USA). 100 μm nylon cell strainer was from Falcon (Corning, NY, USA).
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