P p65 s536 3033

Antibodies for NF-κB p50 (13586, 1:1000), p65 (8242, 1:1000), IκBα (4814, 1:1000), p-p65 (S536) (3033, 1:1000), p-IκBα (Ser32/36) (9246, 1:1000), anti-TNF (6945, 1:1000), and His-Tag (2365, 1:2000) were obtained from Cell Signalling Technology. Anti-hNMI (ab183724, 1:1000), anti-HMGB1 (ab79823, 1:1000), anti-LDHB1 (ab75167, 1:1000), anti-LaminB (ab133741, 1:1000), and anti-N-myc (ab24193, 1:500) antibodies were obtained from Abcam. Anti-β-actin (A5441, 1:10000) antibody was obtained from Sigma-Aldrich. Polyclonal antibody for mNMI (Anti-mNMI, 1:1000) was prepared using recombinant mNMI protein according to previous report^{46 (link)}. Anti-hIFP35 (H00003430-D01P, 1:1000) antibody was obtained from Abnova. The ELISA kits for hNMI (CSB-EL015893HU), mNMI (CSB-EL015893MO), mouse AST (CSB-E12649m), and mouse ALT (CSB-E16539m) were obtained from CUSABIO. The ELISA kits for mIFP35 (E10460m) was purchased from Wuhan EIAab Science Co. The ELISA kits for mouse IL-6 (431305) and mouse TNF (430905) were purchased from Biolegend.
The activity and specificity of anti-hNMI (ab183724), anti-mNMI, anti-hIFP35 (H00003430-D01P), mNMI (CSB-EL015893MO), and mIFP35 (E10460m) ELISA kits were tested as shown in Supplementary Fig. 6.

TNFα and IL-1β were purchased from R&D Systems. ASA was purchased from Sigma. IKKVII was purchased from EMD Millipore. Antibodies for p-IKKα/β (#2697), IKKα (#2682), IKKβ (#2370), p-IκBα (#2859), IκBα (#4814) and p-p65 (S536, #3033) were purchased from Cell Signaling. The antibody for p65 (sc-372) was purchased from Santa Cruz and β-actin (A5441) from Sigma.