Pbr322 plasmid dna

Materials: all reagents and solvents were obtained from commercial sources and used without further purification. [Cu(H2O)(1,10phenanthroline)2](NO3)2, the reference copper-based AMN, was prepared following a reported procedure. [38] The ligand 4'-((naphthalen-2yl)methoxy)-2,2':6',2"-terpyridine (Naphtpy) was synthesized as described earlier. [14] pBR322 plasmid DNA was purchased from Roche and calf thymus DNA was obtained from Sigma-Aldrich.
Instrumentation and methods: All reactions were performed under aerobic conditions. 1 H and 13 C [39] NMR spectra were recorded at room temperature with a Varian Unity 400 MHz spectrometer. Proton and carbon chemical shifts are reported in parts per million (ppm, δ scale) and are referenced to the residual solvent peaks. Infrared spectra (KBr 10.1002/cbic.202000154

All reactions were performed under aerobic conditions and all reagents and solvents were purchased from Sigma-Aldrich or Acros Organics. The reference copper(II)-phenanthroline complex [Cu(H 2 O)(1,10-phenanthroline) 2 ](NO 3 ) 2 was prepared following the procedure described by K. J. Catalan et al. 69 pBR322 plasmid DNA was purchased from Roche and calf thymus DNA was obtained from Sigma-Aldrich. 1 H, 13 C { 1 H} and heteronuclear { 1 H-13 C}-HSQC NMR spectra were recorded at room temperature with a Varian Unity 400 MHz spectrometer. Proton and carbon chemical shifts are expressed in parts per million ( ppm, δ scale) and are referenced to the peak of the solvent used. Infrared spectra (as KBr pellets) were recorded using a Nicolet-5700 FT-IR (in the range 4000-400 cm -1 ), and data are represented as the frequency of absorption (cm -1 ). Elemental analyses were performed by the Servei de Microanalisi, Serveis Cientificotècnics of the University of Barcelona. UV-Vis experiments were performed with a Varian Cary-100 spectrophotometer. The fluorescence measurements were carried out with a KONTRON SFM 25 spectrofluorometer. ESI mass spectroscopy was carried out using a LC/MSD-TOF Spectrometer from Agilent Technologies, equipped with an electrospray ionization (ESI) source at the Serveis Cientificotècnics of the University of Barcelona.