Human osteosarcoma MG63 and normal osteoblast hFOB cell lines were purchased from the Cell Bank Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Dulbecco's modi ed Eagle's medium-F12 (DMEM/F12) was purchased from Hyclone, USA; Fetal Bovine Serum (FBS), Penicillin-streptomycin (100 U/mL) were purchased from Hangzhou Sijiqing Biological Company; RIPA Lysis buffer, BCA Protein Quanti cation Reagent Kit were purchased from Invitrogen, USA; Opti-MEM (Gbico, UK); Anti-Tim-3 monoclonal antibody was obtained from BD Bioscience, USA; Rabbit anti-E-Cadherin, N-cadherin, Vimentin, and GAPDH monoclonal antibodies were purchased from Abcam, USA; Rabbit anti-CD9, CD81, and CD63 monoclonal antibodies were purchased from Santa Cruz, USA; Horseradish peroxidase (HRP) labeled goat anti-rabbit IgG secondary antibody was purchased from Guangzhou Jingcai; PKH67 green uorescent cell linker dye, and PMA were purchased from Sigma Company, USA.
Bca protein quanti cation reagent kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The BCA Protein Quantification Reagent Kit is a colorimetric assay used to quantify the total protein concentration in a sample. The kit utilizes the bicinchoninic acid (BCA) method, which produces a purple-colored reaction that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions)
Anti tim 3 monoclonal antibody, by BD (2 mentions)
Pkh67 green uorescent cell linker dye, by Merck Group (2 mentions)
Opti mem, by BD (2 mentions)
Rabbit anti e cadherin, by Abcam (2 mentions)
Phorbol myristate acetate (pma), by Merck Group (2 mentions)
Vimentin, by Abcam (2 mentions)
N cadherin, by Abcam (2 mentions)
2 protocols using bca protein quanti cation reagent kit
1
In vitro Study of Human Osteosarcoma
2
In vitro Study of Human Osteosarcoma
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Human osteosarcoma MG63 and normal osteoblast hFOB cell lines were purchased from the Cell Bank Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Dulbecco's modi ed Eagle's medium-F12 (DMEM/F12) was purchased from Hyclone, USA; Fetal Bovine Serum (FBS), Penicillin-streptomycin (100 U/mL) were purchased from Hangzhou Sijiqing Biological Company; RIPA Lysis buffer, BCA Protein Quanti cation Reagent Kit were purchased from Invitrogen, USA; Opti-MEM (Gbico, UK); Anti-Tim-3 monoclonal antibody was obtained from BD Bioscience, USA; Rabbit anti-E-Cadherin, N-cadherin, Vimentin, and GAPDH monoclonal antibodies were purchased from Abcam, USA; Rabbit anti-CD9, CD81, and CD63 monoclonal antibodies were purchased from Santa Cruz, USA; Horseradish peroxidase (HRP) labeled goat anti-rabbit IgG secondary antibody was purchased from Guangzhou Jingcai; PKH67 green uorescent cell linker dye, and PMA were purchased from Sigma Company, USA.
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