For the preparation of ASM, the specified quantities of components were dissolved in 20 mL of sterile water: 100 mg mucin from pig stomach (Sigma-Aldrich); 80 mg unsheared salmon sperm DNA (Sigma-Aldrich); 100 mg NaCl; 44 mg KCl; 0.1 mL egg yolk emulsion (Sigma-Aldrich); 0.12 mg diethylentriaminepentaacetic acid (Sigma-Aldrich); 100 mg casamino acids (Gibco); 11.4 mg glucose (Sigma-Aldrich). The pH of the solution was adjusted to 6.8 with HCl [40] (link). Each experiment was conducted using either freshly prepared ASM or ASM that had been stored at 4 • C for a maximum of one week.
Mucin from pig stomach
Manufactured by Merck Group
Mucin from pig stomach is a natural glycoprotein extracted from the lining of pig's stomach. It serves as a key component in various research and development applications, providing a reliable source of mucin for further analysis and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using mucin from pig stomach
1
Artificial Saliva Media Preparation
2
Artificial Sputum Medium for CF Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Artificial sputum medium (ASM) was used to mimic the sputum of CF patients, and it was prepared as described by Sriramulu et al. (2005 (link)). Briefly, 5 g/L of mucin from pig stomach (Sigma-Aldrich), 4 g/L of DNA from salmon sperm (Sigma-Aldrich), 5.9 mg/L of diethylenetriaminepentaacetic acid (DTPA, Sigma-Aldrich), 5 g/L of NaCl, 2.2 g/L of KCl, and 5 g/L of casoamino acids (AMESRO) were resuspended in water and the pH adjusted to 7 with Tris base. This ASM was then sterilized in an autoclave at 110°C for 15 min, and after being cooled, 5 mL of egg yolk emulsion (Fluka) was added.
3
Artificial Sputum Medium for CF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Artificial sputum medium (ASM) was used to mimic the sputum of CF patients and it was prepared as described by Sriramulu et al. (2005) . Briefly, 5 g/L of mucin from pig stomach (Sigma-Aldrich), 4 g/L of DNA from salmon sperm (Sigma-Aldrich), 5.9 mg/L of diethylene triamine pentaacetic acid (DTPA, Sigma-Aldrich), 5 g/L of NaCl, 2.2 g/L of KCl and 5 g/L of casoamino acids (AMESRO) were resuspended in water and the pH adjusted to 7 with Tris base. This ASM was then sterilized in an autoclave at 110 °C for 15 min and, after cooled, 5 mL of egg yolk emulsion (Fluka) were added.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!