The following histology solutions were generated in house: Weigert's iodine [2 g potassium iodide (03124, Sigma-Aldrich), 1 g iodine (326143, Sigma-Aldrich), 100 ml distilled water]; Verheoff's solution [20 ml 5% alcoholic Haematoxylin (H3136, Sigma-Aldrich), 8 ml 10% ferric chloride (157740, Sigma-Aldrich), 8 ml Weigert's iodine]; Van Gieson's solution [5 ml aqueous acid fuschin (F8129, Sigma-Aldrich), 100 ml saturated aqueous picric acid (84512.260, VWR)]; Picrosirius Red solution [0.5 g Direct Red 80 (365548, Sigma-Aldrich), 500 ml saturated aqueous picric acid (84512.260, VWR)]; Weigert's Haematoxylin (1:1 ratio of Weigert's solution A and Weigert's solution B); Weigert's solution A [1% Haematoxylin (H3136, Sigma-Aldrich) in 100% ethanol]; Weigert's solution B [4 ml 30% ferric chloride (157740, Sigma-Aldrich), 1 ml 12 N hydrochloric acid, 95 ml water]; acidified water (5 ml glacial acetic acid, 1 l distilled water); acid/alcohol solution (70% ethanol, 0.1% hydrochloric acid).
Haematoxylin h3136
Manufactured by Merck Group
Haematoxylin (H3136) is a natural dye commonly used as a staining agent in histology and cytology laboratories. It is extracted from the heartwood of the Logwood tree (Haematoxylum campechianum). Haematoxylin serves as a nuclear stain, coloring cell nuclei blue or purple, which allows for the visualization and identification of cellular structures during microscopic examination.
Automatically generated - may contain errors
Lab products found in correlation
Ferric chloride, by Merck Group (1 mentions)
Direct red 80, by Merck Group (1 mentions)
Potassium iodide, by Merck Group (1 mentions)
Iodine, by Merck Group (1 mentions)
Haematoxylin, by Merck Group (1 mentions)
Neutral balsam, by Maclin Biochemical Technology (1 mentions)
N861409, by Maclin Biochemical Technology (1 mentions)
Primotech, by Zeiss (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
2 protocols using haematoxylin h3136
1
Histology Solutions for Tissue Analysis
2
Histopathological Analysis of Rat Liver and Intestine
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After rat liver and intestinal mucosa were fixed in 4% paraformaldehyde (16005, Sigma-Aldrich, St. Louis, MO) for 24 h, they were dehydrated by gradient alcohol, transparentized by xylene (95682, Sigma-Aldrich, St. Louis, MO), and embedded in paraffin (1496904, Sigma-Aldrich, St. Louis, MO). Subsequently, the paraffinized tissues were cut into 5 μm thick sections, dewaxed by xylene and rehydrated by gradient alcohol. Then, the sections were stained with haematoxylin (H3136, Sigma-Aldrich, St. Louis, MO) for 12 min. After being differentiated by hydrochloric alcohol, the sections were stained with eosin (E4009, Sigma-Aldrich, St. Louis, MO) for 5 min. Thereafter, the stained sections were sealed by neutral balsam (N861409, Macklin, Shanghai, China) and dried at 37 °C for 4 h. Afterwards, the histopathological changes in the liver tissues and intestinal mucosa tissues were observed by an optical microscope (ZEISS Primotech, Carl Zeiss, Oberkochen, Germany) under magnifications of ×200 and ×40, respectively.
The morphological changes in liver were determined by NAFLD activity score, which includes histological features and has been defined as unweighted sum of scores for steatosis (0–3), lobular inflammation (0–3) and ballooning (0–2).
The morphological changes in liver were determined by NAFLD activity score, which includes histological features and has been defined as unweighted sum of scores for steatosis (0–3), lobular inflammation (0–3) and ballooning (0–2).
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