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2 protocols using anti phospho nrf2 antibody

1

Antioxidant and Anti-apoptotic Mechanisms

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Tricarbonyldichlororuthenuim (II) dimer (CORM-2), 6-hydroxydopamine hydrochloride, MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], anti-actin antibody and other chemicals were provided from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) were obtained from Hyclone (GE Healthcare life sciences, Logan, Utah, USA). Fetal bovine serum (FBS) and penicillin-streptomycin antibiotic were purchased from Gibco BRL (Grand Island, NY, USA). Anti-bodies against Bcl-2, Bax, capase-3, NF-E2-related factor 2 (Nrf2), manganese superoxide dismutase (MnSOD) and copper-zinc superoxide dismutase (CuZnSOD) were provided from Santa cruz Biotechnology (Santa Cruz, CA, USA). Anti-heme oxygenase-1 (HO-1) antibody was supplied by Enzo life sciences (Farmingdale, NY, USA) and Anti-phospho Nrf2 antibody was supplied by Abcam (Cambridge, UK). Anti-cleaved caspase-3 was purchased from Cell signaling (Danvers, MA, USA).
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2

Antibody Sourcing and Chemical Reagents

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Anti-acetylated-α-tubulin, anti-SOD-1, and anti-rabbit HRP-labeled secondary antibodies were obtained from Santa Cruz (SC-23950, SC-11407, and SC-2004; Heidelberg, Germany). Anti-GAPDH antibody was obtained from Sigma-Aldrich (G9545; Munich, Germany). Anti-phospho-p38 MAPKinase and anti-catalase antibodies were obtained from Cell Signaling (Frankfurt am Main, Germany). Anti-phospho-Nrf2 antibody was obtained from Abcam (ab76026; Cambridge, United Kingdom). Alexa Fluor 448-labeled secondary antibody was obtained from Invitrogen (Karlsruhe, Germany). N-Acetylcysteine (NAC) and nicotine were obtained from Carl Roth (Karlsruhe, Germany). L-Ascorbic acid was obtained from Sigma-Aldrich (Darmstadt, Germany). Cotinine was obtained from Alfa Aesar (Karlsruhe, Germany).
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