The morphology of the nonwovens was investigated by high-resolution scanning electron microscopy (SEM). The SEM micrographs were recorded using a MIRA3 scanning electron microscope (TESCAN, Brno, Czechia). The nonwoven samples were placed on the stub and, to avoid charging problems, fibers were coated with Pd/Au for 30 s in an argon environment using a Q150T ES coater with turbomolecular pump (Quorum Technologies, Lewes, UK).
Turbomolecular pump
Manufactured by Quorum Technologies
Sourced in United Kingdom
A turbomolecular pump is a type of vacuum pump that uses a rapidly spinning rotor to create a high vacuum. It is designed to remove gases from a sealed container, reducing the pressure inside. The rotor spins at high speeds, typically between 20,000 and 90,000 revolutions per minute, to efficiently transfer momentum to gas molecules, thereby evacuating the chamber.
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Lab products found in correlation
2 protocols using turbomolecular pump
1
Nonwoven Fiber Morphology Analysis
2
Scanning Electron Microscopy of Callus
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Several pieces of callus collected after 18 day of culture were analyzed via scanning electron microscopy. The samples were frozen in liquid nitrogen and then freeze-dried, but chemical fixation method was not applied to avoid occurrence of artifacts (Konieczny et al. 2005) . The specimens were covered with a carbon coating using Q150T sputter with turbomolecular pump from Quorum Technologies company. All the observations were done by scanning electron microscope from Hitachi TM-3000 company with EDS system.
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