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Cucurbit 6 uril hydrate

Manufactured by Merck Group
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Cucurbit[6]uril hydrate is a macrocyclic compound consisting of six glycoluril units linked by methylene bridges. It is a symmetrical, barrel-shaped molecule that can form inclusion complexes with a variety of guest molecules. The core function of Cucurbit[6]uril hydrate is to serve as a host molecule in supramolecular chemistry and nanotechnology applications.

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Lab products found in correlation

Micro bio spin p 6 gel column, by Bio-Rad (2 mentions) Acetonitrile, by Merck Group (2 mentions) Milli q water purification system, by Merck Group (2 mentions) Tris hydrochloride, by Merck Group (2 mentions) 4 2 hydroxyethyl piperazine 1 ethanesulfonic acid, by Merck Group (2 mentions) Ascorbic acid, by Merck Group (2 mentions) α hl from staphylococcus aureus, by Merck Group (2 mentions) Hct116, by American Type Culture Collection (1 mentions) Methylene blue, by Merck Group (1 mentions) Sodium acetate, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Sodium dodecyl sulfate (sds), by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Rhodamine b, by Merck Group (1 mentions) Ethylene glycol, by Merck Group (1 mentions) Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions) Methyl blue, by Merck Group (1 mentions) Iron chloride hexahydrate, by Merck Group (1 mentions) Magnesium chloride, by Merck Group (1 mentions)

3 protocols using cucurbit 6 uril hydrate

1

Colorectal Cancer Cell Line Study

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Calcined DE, iron chloride hexahydrate (FeCl3.6H2O), sodium acetate (NaAc), ethylenediamine (EDA), ethylene glycol (EG), cucurbit[6]uril hydrate, sodium dodecyl sulfate (SDS), sodium chloride (NaCl), calcium chloride (CaCl2), magnesium chloride (MgCl2), Methyl Blue, Rhodamine B, Methylene Blue, Thiazolyl blue tetrazolium bromide (MTT), and zinc phthalocyanine (ZnPC) were purchased from Sigma Aldrich (Saint Louis, Missouri, USA). Trypan Blue stain (0.4%), Dulbecco’s modified Eagle’s medium (DMEM, 1×), fetal bovine serum (FBS), and phosphate-buffered saline (PBS) were obtained from Gibco (Big Cabin, Oklahoma, USA). Dimethyl sulfoxide (DMSO) was acquired from EMD Millipore Corp (Darmstadt, Germany). The human colorectal carcinoma cell line HCT-116 (ATCC®CCL-247) was purchased from ATCC (Manassas, VA, USA). All materials were used as received with no further treatment.
Luan Y., Liu H., Qiao Z., Koo B., Shin J., Jang Y.O., Noh J.S, & Shin Y. (2020). An enhanced recyclable 3D adsorbent for diverse bio-applications using biocompatible magnetic nanomulberry and cucurbituril composites. Scientific Reports, 10, 443.
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2

Alkyne-modified DNA Probe Synthesis

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The DNA probe 5′-CCCCCCCCCCT*CCCCCCCCCC-3′ (T*—alkyne-modified thymine) was customized by Sangon Biotechnology Co. Ltd. (Shanghai). Micro Bio-Spin P6 gel columns (Tris buffer) were purchased from Bio-Rad (Hercules, CA). Cu(NO3)2 (99%), KCl (99.99%), NaCl (99.99%), LiCl (99.99%), TRIS hydrochloride (Tris·HCl), Azide Adamantane (97%), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, ≥99.5%), (Ethylenedinitrilo) tetraacetic acid (EDTA, ≥99%), Ascorbic acid (≥99%), Acetonitrile (99.8%), Cucurbit[6]uril hydrate, α-HL from Staphylococcus aureus (lyophilized powder, Protein ~60% by Lowry, ≥10,000 units/mg protein) were purchased from Sigma-Alrich. All working solutions were prepared using deionized water from a Milli-Q water purification system (resistivity 18.2 MΩ/cm, 25 °C, Millipore Corporation) and were filtered through 0.02 μm filters before use.
Wei X., Zhang Z., Wang X., Lenhart B., Gambarini R., Gray J, & Liu C. (2020). Insight into the effects of electrochemical factors on host-guest interaction induced signature events in a biological nanopore. Nanotechnology and Precision Engineering, 3(1), 2-8.
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3

Alkyne-modified DNA Probe Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The DNA probe 5′-CCCCCCCCCCT*CCCCCCCCCC-3′ (T*—alkyne-modified thymine) was customized by Sangon Biotechnology Co. Ltd. (Shanghai). Micro Bio-Spin P6 gel columns (Tris buffer) were purchased from Bio-Rad (Hercules, CA). Cu(NO3)2 (99%), KCl (99.99%), NaCl (99.99%), LiCl (99.99%), TRIS hydrochloride (Tris·HCl), Azide Adamantane (97%), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, ≥99.5%), (Ethylenedinitrilo) tetraacetic acid (EDTA, ≥99%), Ascorbic acid (≥99%), Acetonitrile (99.8%), Cucurbit[6]uril hydrate, α-HL from Staphylococcus aureus (lyophilized powder, Protein ~60% by Lowry, ≥10,000 units/mg protein) were purchased from Sigma-Alrich. All working solutions were prepared using deionized water from a Milli-Q water purification system (resistivity 18.2 MΩ/cm, 25 °C, Millipore Corporation) and were filtered through 0.02 μm filters before use.
Wei X., Zhang Z., Wang X., Lenhart B., Gambarini R., Gray J, & Liu C. (2020). Insight into the effects of electrochemical factors on host-guest interaction induced signature events in a biological nanopore. Nanotechnology and Precision Engineering, 3(1), 2-8.
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