The largest database of trusted experimental protocols

Gill s hematoxylin solution

Manufactured by Fujifilm
Sourced in Japan

Gill's hematoxylin solution is a laboratory reagent used for staining biological samples. It is a standardized formulation of hematoxylin, a natural dye derived from the logwood tree. The primary function of Gill's hematoxylin solution is to selectively stain the nuclei of cells, providing a visual contrast that aids in microscopic examination and analysis.

Automatically generated - may contain errors

2 protocols using gill s hematoxylin solution

1

Immunohistochemical Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antigen retrieval was performed by autoclave treatment at 100℃ for 10 min using Target retrieval solution High P.H. (DAKO Cytomation, Kyoto, Japan). At rst, GST-pi polyclonal antibody (MBL, Nagoya, Japan), COX-2 polyclonal antibody (Cayman Chemical, Chicago, IL), P53 polyclonal antibody (FL393) (Santa Cruz, Santacruz, CA), and Smad4 monoclonal antibody (B-8) (Santa Cruz) was used. Proteins were identi ed with Envision kit (DAKO cytomation, Kyoto, Japan). They were colored by 3,3-Diaminobenzidine Tetrahydrochloride (DAB) (WAKO, Osaka, Japan), and their nuclei were stained by Gill's hematoxylin solution (WAKO). It was determined to be positive when more than 50% of the composing cells was stained.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antigen retrieval was performed by autoclave treatment at 100℃ for 10 min using Target retrieval solution High P.H. (DAKO Cytomation, Kyoto, Japan). At rst, GST-pi polyclonal antibody (MBL, Nagoya, Japan), COX-2 polyclonal antibody (Cayman Chemical, Chicago, IL), P53 polyclonal antibody (FL393) (Santa Cruz, Santacruz, CA), and Smad4 monoclonal antibody (B-8) (Santa Cruz) was used. Proteins were identi ed with Envision kit (DAKO cytomation, Kyoto, Japan). They were colored by 3,3-Diaminobenzidine Tetrahydrochloride (DAB) (WAKO, Osaka, Japan), and their nuclei were stained by Gill's hematoxylin solution (WAKO). It was determined to be positive when more than 50% of the composing cells was stained.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!