Antigen retrieval was performed by autoclave treatment at 100℃ for 10 min using Target retrieval solution High P.H. (DAKO Cytomation, Kyoto, Japan). At rst, GST-pi polyclonal antibody (MBL, Nagoya, Japan), COX-2 polyclonal antibody (Cayman Chemical, Chicago, IL), P53 polyclonal antibody (FL393) (Santa Cruz, Santacruz, CA), and Smad4 monoclonal antibody (B-8) (Santa Cruz) was used. Proteins were identi ed with Envision kit (DAKO cytomation, Kyoto, Japan). They were colored by 3,3-Diaminobenzidine Tetrahydrochloride (DAB) (WAKO, Osaka, Japan), and their nuclei were stained by Gill's hematoxylin solution (WAKO). It was determined to be positive when more than 50% of the composing cells was stained.
Gill s hematoxylin solution
Manufactured by Fujifilm
Sourced in Japan
Gill's hematoxylin solution is a laboratory reagent used for staining biological samples. It is a standardized formulation of hematoxylin, a natural dye derived from the logwood tree. The primary function of Gill's hematoxylin solution is to selectively stain the nuclei of cells, providing a visual contrast that aids in microscopic examination and analysis.
Automatically generated - may contain errors
Lab products found in correlation
P53 polyclonal antibody fl393, by Santa Cruz Biotechnology (2 mentions)
Cox 2 polyclonal antibody, by Cayman Chemical (2 mentions)
Smad4 monoclonal antibody b 8, by Santa Cruz Biotechnology (2 mentions)
3 3 diaminobenzidine tetrahydrochloride dab, by Fujifilm (2 mentions)
Envision kit, by Agilent Technologies (2 mentions)
2 protocols using gill s hematoxylin solution
1
Immunohistochemical Analysis of Protein Expression
2
Immunohistochemical Analysis of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antigen retrieval was performed by autoclave treatment at 100℃ for 10 min using Target retrieval solution High P.H. (DAKO Cytomation, Kyoto, Japan). At rst, GST-pi polyclonal antibody (MBL, Nagoya, Japan), COX-2 polyclonal antibody (Cayman Chemical, Chicago, IL), P53 polyclonal antibody (FL393) (Santa Cruz, Santacruz, CA), and Smad4 monoclonal antibody (B-8) (Santa Cruz) was used. Proteins were identi ed with Envision kit (DAKO cytomation, Kyoto, Japan). They were colored by 3,3-Diaminobenzidine Tetrahydrochloride (DAB) (WAKO, Osaka, Japan), and their nuclei were stained by Gill's hematoxylin solution (WAKO). It was determined to be positive when more than 50% of the composing cells was stained.
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