Hpa007041

Manufactured by Atlas Antibodies

HPA007041 is a recombinant antibody produced in human cells. It is designed to detect the CEACAM5 protein, also known as carcinoembryonic antigen (CEA). This antibody can be used for various research applications involving the detection and analysis of CEACAM5 expression.

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Lab products found in correlation

Rabbit antihuman tau a0024 polyclonal antibody, by Agilent Technologies (2 mentions) Mouse monoclonal anti gad67 antibody, by Merck Group (1 mentions)

2 protocols using hpa007041

Immunohistochemical Analysis of Arteriolar ALK1

Cited 1 time

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We analyzed arteriolar ALK1 using rabbit polyclonal anti-ALK1 antibody (1:25, HPA007041,
Atlas Antibodies, Stockholm, Sweden) with previously characterized specificity (Adams et al. 2018 (link)). Mouse antihuman muscle specific
actin (MSA; also known as alpha smooth muscle actin α-SMA) [HHF35] monoclonal antibody
(0.22 μg/mL, Marque Corporation, Rocklin, CA) highlighted vascular walls in order to help
select arterioles for analysis. Mouse antihuman amyloid-β (Aβ) [6F/3D] monoclonal antibody
(1:25, Dako, Glostrup, Denmark) rabbit antihuman tau [A0024] polyclonal antibody (1:3200,
Dako, Glostrup, Denmark), and mouse antihuman phospho-PHF-tau [AT8] monoclonal antibody
(1:2000, Pierce, Rockford, IL) were also used for confirmation of neuropathological report
data and qualitative analyses.

Anderson K.E., Bellio T.A., Aniskovich E., Adams S.L., Blusztajn J.K, & Delalle I. (2020). The Expression of Activin Receptor-Like Kinase 1 (ACVRL1/ALK1) in Hippocampal Arterioles Declines During Progression of Alzheimer’s Disease. Cerebral Cortex Communications, 1(1), tgaa031.

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Immunohistochemical Characterization of ALK1

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Primary antibodies included rabbit polyclonal anti-ALK1 (1:25, HPA007041, Atlas Antibodies). This anti-ALK1 antibody immunohistochemical specificity in FFPE tissues was characterized by immunoabsorption assay with the ALK1 immunogen (APrEST71390, Atlas Antibodies) incubated at 5X molar excess of the ALK1 antibody at room temperature for 1 h before applying primary and immunogen or primary alone to control tissue in the identical IHC protocol used for ALK1 analysis (Supplementary Fig. 1A-D). Specificity of this antibody was further shown in western blot analysis of mouse hippocampus, revealing a single band at 70 kDa, corresponding to ALK1 signal (Supplementary Fig. 1E). Mouse anti-GAD67 monoclonal antibody (1:125, GR419, Sigma, Dallas, TX), mouse anti-human amyloid-β (Aβ) [6F/3D] monoclonal antibody (1:25, Dako, Glostrup, Denmark) rabbit anti-human tau [A0024] polyclonal antibody (1:3200, Dako), and mouse anti-human phospho-PHF-tau [AT8] monoclonal antibody (1:2000, Pierce) were also used for confirmation of neuropathological report data and qualitative analyses.

Adams S.L., Benayoun L., Tilton K., Mellott T.J., Seshadri S., Blusztajn J.K, & Delalle I. (2018). Immunohistochemical Analysis of Activin Receptor-Like Kinase 1 (ACVRL1/ALK1) Expression in the Rat and Human Hippocampus: Decline in CA3 During Progression of Alzheimer’s Disease. Journal of Alzheimer's disease : JAD, 63(4), 1433-1443.

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