HA immunoprecipitation was done as previously described49 (link). Cells were washed once with ice-cold PBS and harvested in immunoprecipitation buffer (20 mM HEPES pH 7.9, 200 mM NaCl, 0.5 mM EDTA, 10% glycerol, 0.2% NP-40) supplemented with phosphatase and protease inhibitors (Sigma), lysed for 30 min at 4 °C and cleared by centrifugation. For immunoprecipitation, HA-coupled magnetic beads (Pierce Thermo Fisher Scientific) were washed with IP buffer, added to the lysate and incubated for 3 h at 4 °C. Elution was performed in 40 µl 1x LDS buffer (NuPAGE Thermo Fisher Scientific) by incubating at 37 °C with shaking for 30 min.
Ha coupled magnetic beads
Manufactured by Thermo Fisher Scientific
HA-coupled magnetic beads are paramagnetic particles with covalently coupled hyaluronic acid (HA). They are designed for various biomedical and biochemical applications that require magnetic separation and HA-based interactions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ha coupled magnetic beads
1
HA-Immunoprecipitation Protocol for Protein Isolation
2
HA-Immunoprecipitation Protocol for Protein Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HA immunoprecipitation was done as previously described49 (link). Cells were washed once with ice-cold PBS and harvested in immunoprecipitation buffer (20 mM HEPES pH 7.9, 200 mM NaCl, 0.5 mM EDTA, 10% glycerol, 0.2% NP-40) supplemented with phosphatase and protease inhibitors (Sigma), lysed for 30 min at 4 °C and cleared by centrifugation. For immunoprecipitation, HA-coupled magnetic beads (Pierce Thermo Fisher Scientific) were washed with IP buffer, added to the lysate and incubated for 3 h at 4 °C. Elution was performed in 40 µl 1x LDS buffer (NuPAGE Thermo Fisher Scientific) by incubating at 37 °C with shaking for 30 min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!