Spherical c18 100a reversed phase silica gel rp 18

Manufactured by Silicycle

Sourced in Canada

Spherical C18 100A Reversed Phase Silica Gel (RP-18) is a type of silica-based chromatographic material used in reversed-phase liquid chromatography. It has a particle size of 60-100 mesh and a pore size of 100 angstroms. The material is designed for the separation and purification of a variety of organic compounds.

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Lab products found in correlation

Apex 2 mass spectrometer, by Bruker (8 mentions) Spherical c18 column, by Waters Corporation (7 mentions) Uv 240 spectrophotometer, by Jasco (7 mentions) Dip 370 polarimeter, by Jasco (7 mentions) 2000 ft ir spectrophotometer, by PerkinElmer (6 mentions) Unity plus 400, by Agilent Technologies (5 mentions) Precoated silica gel 60 f254 plates, by Merck Group (5 mentions) Silica gel 60, by Merck Group (5 mentions) Mercury 500, by Agilent Technologies (4 mentions) Vnmrs 600, by Agilent Technologies (3 mentions) Ft ir 4200 spectrophotometer, by Jasco (1 mentions) V 530 uv vis spectrophotometer, by Jasco (1 mentions) P 2000 polarimeter, by Jasco (1 mentions) Mercury 400, by Agilent Technologies (1 mentions) F254 precoated plates, by Merck Group (1 mentions) Ftir spectrophotometer, by PerkinElmer (1 mentions)

8 protocols using spherical c18 100a reversed phase silica gel rp 18

Analytical Methods for Natural Product Research

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For the TLC, we used silica gel 60 F254-precoated plates (Merck); for column chromatography (CC), we used silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC analysis, we used a spherical C18 column (250 × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, with λmax (log ε) in nm. For optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer, with ν in cm⁻¹. For the ¹H-, ¹³C-, and 2D-NMR spectra, we used Varian-VNMRS-600 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.

Cheng M.J., Chen J.J., Wu M.D., Leu J.Y, & Tseng M. (2023). Antifungal Activities of Compounds Produced by Newly Isolated Acrocarpospora Strains. Antibiotics, 12(1), 95.

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Analytical Methods for Chemical Characterization

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General. TLC: silica gel 60 F₂₅₄ precoated plates (Merck, Kenilworth, NJ, USA). Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). UV Spectra: Jasco V-530 UV/VIS spectrophotometer (Tokyo, Japan); λ_max (log ε) in nm. Optical rotation: Jasco P-2000 polarimeter; in CHCl₃. IR Spectra: Jasco FTIR-4200 spectrophotometer; ν in cm⁻¹. ¹H, ¹³C and 2D NMR spectra: Varian-VNMRS-600 (Palo Alto, CA, USA), and Varian-Mercury-400 spectrometers; δ in ppm rel. to Me₄Si, J in Hz. EI–MS: VG-Biotech Quatro-5022 mass spectrometer (Palo Alto, CA, USA); m/z (rel. %). ESI and HRESIMS: Bruker APEX-II mass spectrometer (Billerica, MA, USA); in m/z.

Chang H.S., Lin C.H., Chen Y.S., Wang H.C., Chan H.Y., Hsieh S.Y., Wu H.C., Cheng M.J., Yuan G.F., Lin S.Y., Lin Y.J, & Chen I.S. (2016). Secondary Metabolites of the Endophytic Fungus Lachnum abnorme from Ardisia cornudentata. International Journal of Molecular Sciences, 17(9), 1512.

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Analytical Techniques for Natural Product Isolation

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TLC: silica gel 60 F₂₅₄ precoated plates (Merck, Darmstadt, German). Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle, Quebec, Canada). HPLC: Spherical C18 column (250 × 10 mm, 5 μm) (Waters, Milford, MA, USA); LDC-Analytical-III apparatus. UV Spectra: Jasco UV-240 spectrophotometer; λ_max (log ε) in nm. Optical rotation: Jasco DIP-370 polarimeter; in CHCl₃. IR Spectra: Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm⁻¹. ¹H-, ¹³C- and 2D-NMR spectra were obtained utilizing a Varian-Mercury-500 and Varian-Unity-Plus-400 instrument and reported in CDCl₃. ¹H and ¹³CNMR chemical shifts are reported in ppm relative to either TMS (¹H) (δ = 0 ppm, J in Hz) as an internal standard or the residual solvent peak as following: CDCl₃ = 7.26 (¹H NMR), CDCl₃ = 77.0 (¹³C NMR). ESI and HRESIMS: Bruker APEX-II mass spectrometer; in m/z.

Cheng M.J., Wu M.D., Chang C.L., Chang H.S., Chyu C.F, & Kuo Y.H. (2022). Novel Antifungal Dimers from the Roots of Taiwania cryptomerioides. Molecules, 27(2), 437.

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Comprehensive Analytical Techniques for Compound Characterization

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TLC: silica gel 60 F₂₅₄ precoated plates (Merck, Darmstadt, Germany). Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (SiliCycle, Quebec City, Canada). HPLC: Spherical C18 column (250 mm × 10 mm, 5μm) (Waters, Milford, MA, USA); LDC-Analytical-III apparatus. UV Spectra: Jasco UV-240 spectrophotometer; λ_max (log ε) in nm. Optical rotation: Jasco DIP-370 polarimeter; in CHCl₃. IR Spectra: Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm⁻¹. ¹H-, ¹³C-, and 2D-NMR spectra: Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers; δ in ppm rel. to Me₄Si, J in Hz. ESI and HRESIMS: Bruker APEX-II mass spectrometer; in m/z.

Wu M.D., Cheng M.J., Chen J.J., Khamthong N., Lin W.W, & Kuo Y.H. (2022). Secondary Metabolites with Antimicrobial Activities from Chamaecyparis obtusa var. formosana. Molecules, 27(2), 429.

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Analytical Techniques for Chemical Characterization

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TLC: silica gel 60 F₂₅₄ precoated plates (Merck). Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). HPLC: Spherical C18 column (250 × 10 mm, 5μm) (Waters); LDC-Analytical-III apparatus. UV Spectra: Jasco UV-240 spectrophotometer; λ_max (log ε) in nm. Optical rotation: Jasco DIP-370 polarimeter; in CHCl₃. IR Spectra: Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm⁻¹. ¹H-, ¹³C- and 2D-NMR spectra: Varian-Mercury-400 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me₄Si, J in Hz. ESI and HRESIMS: Bruker APEX-II mass spectrometer; in m/z.

Su Y.S., Chen J.J., Cheng M.J., Chai C.Y., Kwan A.L., Huang J.C, & Kuo Y.H. (2021). Saccharpiscinols A–C: Flavans with Potential Anti-Inflammatory Activities from One Actinobacteria Saccharomonospora piscinae. Molecules, 26(16), 4909.

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Analytical Techniques for Chemical Characterization

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For the TLC, we used silica gel 60 F₂₅₄ precoated plates (Merck); for column chromatography (CC), silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC, we used a spherical C18 column (250 × 10 mm, 5μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, λ_max (log ε) in nm. For the optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl₃. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm⁻¹. For the ¹H-, ¹³C- and 2D-NMR spectra, we used Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers; δ in ppm rel. to Me₄Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.

Wu M.D, & Cheng M.J. (2022). Undescribed Metabolites from an Actinobacteria Acrocarpospora punica and Their Anti-Inflammatory Activity. Molecules, 27(22), 7982.

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Detailed Chromatographic Techniques for Compound Separation

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For TLC, we used silica gel 60 F₂₅₄ precoated plates (Merck); for column chromatography (CC), silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For HPLC, we used spherical C18 column (250 × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, λ_max (log ε) in nm. For the optical rotation, we used Jasco DIP-370 polarimeter, in CHCl₃. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm⁻¹. For the ¹H-, ¹³C- and 2D-NMR spectra, we used Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers; δ in ppm rel. to Me₄Si, J in Hz. For ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.

Cheng M.J., Wu M.D., Chen J.J., Su Y.S, & Kuo Y.H. (2021). Secondary Metabolites with Antimycobacterial Activities from One Actinobacteria: Herbidospora yilanensis. Molecules, 26(20), 6236.

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Analytical Methods for Compound Characterization

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For the TLC, we used silica gel 60 F254-precoated plates (Merck); for column chromatography (CC), we used silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC analysis, we used a spherical C18 column (250 mm × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, with λmax (log ε) in nm. For optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer, with ν in cm⁻¹. For the 1H-, 13C-, and 2D-NMR spectra, we used Varian-VNMRS-600 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.

Wu M.D., Chen J.J, & Cheng M.J. (2023). Secondary Metabolites with Antifungal Activities from Mangrove Derived Fungus Monascus purpureus WMD2424. Marine Drugs, 21(4), 200.

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