Cellometer mini automatic cell counter

Manufactured by Revvity

Sourced in United States

The Cellometer-Mini Automatic Cell Counter is a compact and automated cell counting device. It can rapidly and accurately determine the concentration and viability of cells in a sample.

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Lab products found in correlation

Accutase, by Thermo Fisher Scientific (2 mentions) Matrigel, by Corning (1 mentions)

Close spelling variants of this product

Cellometer Automatic Cell Counter Cellometer Mini Cell Counter Cellometer cell counter Automatic cell counter Cellometer Mini Cell counter Cellometer

3 protocols using cellometer mini automatic cell counter

Quantifying HUVEC Tube Formation in Matrigel

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Matrigel tube formation assay was used to determine HUVECs tube formation capacity. Matrigel (Corning, USA) was thawed at 4°C overnight. The liquid Matrigel was spread into 96-well plates (50 μL per well) and incubated in a 5% CO₂ incubator at 37°C for 40 minutes. Cell suspensions were counted using a Cellometer-Mini Automatic Cell Counter (Nexcelom Bioscience, Lawrence, MA, USA). HUVECs (5 × 10³ per well) were seeded on the Matrigel-coated plates and incubated for 6 hours. The network formation in Matrigel was observed using an inverted light microscope. The Image J software plugin “Angiogenesis Analyzer” quantified the extent of tube formation before statistical analysis.

Li H., Yang Z., Liang W., Nie H., Guan Y., Yang N., Ji T., Liu Y., Huang Y., Zhang L., Yan J, & Zhang C. (2024). DHCR24 Insufficiency Promotes Vascular Endothelial Cell Senescence and Endothelial Dysfunction via Inhibition of Caveolin-1/ERK Signaling. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 79(4), glae059.

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Cell Dissociation and Counting

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Cells were dissociated using Accutase^® (ThermoFisher Scientific) and counted using Trypan blue exclusion method and a Cellometer-Mini Automatic Cell Counter (Nexcelom). Cell counting was performed before the seeding of scaffolds and test groups used in all the additionally described studies.

Lawrence L.M., Salary R.(., Miller V., Valluri A., Denning K.L., Case-Perry S., Abdelgaber K., Smith S., Claudio P.P, & Day J.B. (2023). Osteoregenerative Potential of 3D-Printed Poly ε-Caprolactone Tissue Scaffolds In Vitro Using Minimally Manipulative Expansion of Primary Human Bone Marrow Stem Cells. International Journal of Molecular Sciences, 24(5), 4940.

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Dissociation and Cell Counting

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Cells were dissociated using Accutase^® (ThermoFisher Scientific) and counted using Trypan blue exclusion method and a Cellometer-Mini Automatic Cell Counter (Nexcelom).

Lawrence L.M., Cottrill A., Valluri A., Marenzi G., Denning K.L., Valluri J., Claudio P.P, & Day J.B. (2019). Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects. International Journal of Molecular Sciences, 20(3), 612.

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