Alltype kit

Manufactured by Thermo Fisher Scientific

Sourced in Netherlands

The ALLType kit is a laboratory equipment product designed for performing molecular diagnostic tests. It provides a comprehensive solution for nucleic acid extraction, amplification, and detection. The kit includes reagents and consumables required for these processes.

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Lab products found in correlation

Miseq sequencer, by Illumina (1 mentions) Labtype kit, by Thermo Fisher Scientific (1 mentions)

2 protocols using alltype kit

HLA Genotyping Using Multiplex PCR

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For most donors HLA genotyping was performed using the One Lambda ALLType kit, which uses multiplex PCR to amplify full HLA-A/B/C gene sequences, and from exon 2 to the 3′ UTR of the HLA-DRB1/3/4/5/DQB1 genes. Prepared libraries were run on an Illumina MiSeq sequencer using standard flow-cell with 2 × 150 paired-end sequencing. Reads were analyzed using the One Lambda HLA TypeStream Visual Software (TSV), version 2.0.0.27232 and the IPD-IMGT/HLA database 3.39.0.0. Other donors were HLA genotyped by Sanger sequencing for loci HLA-A, B, C, DRB1 and DQB1, using Protrans S4 and Protrans S3 reagents, respectively. The PCR products were prepared for sequencing with BigDye Terminator v1.1. Capillary electrophoresis was performed on a Genetic Analyzer Nanophore05. One donor’s HLA genotyping was determined based on existing exome sequencing data.

Shomuradova A.S., Vagida M.S., Sheetikov S.A., Zornikova K.V., Kiryukhin D., Titov A., Peshkova I.O., Khmelevskaya A., Dianov D.V., Malasheva M., Shmelev A., Serdyuk Y., Bagaev D.V., Pivnyuk A., Shcherbinin D.S., Maleeva A.V., Shakirova N.T., Pilunov A., Malko D.B., Khamaganova E.G., Biderman B., Ivanov A., Shugay M, & Efimov G.A. (2020). SARS-CoV-2 Epitopes Are Recognized by a Public and Diverse Repertoire of Human T Cell Receptors. Immunity, 53(6), 1245-1257.e5.

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High-Resolution HLA Typing by NGS and Sanger

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HLA-A, B, C, and DRB1 were typed at high resolution level by next generation sequencing (ALLType kit, One Lambda, West Hills, CA) or the combination of sequence-specific oligonucleotide probe (LabType kit, One Lambda) and Sanger sequence-based typing kit (SBTexcellerator kit, GenDx, Utrecht, Netherlands).

Jia B., Zhao C., Bayerl M., Shike H., Claxton D.F., Ehmann W.C., Mineishi S., Schell T.D., Zheng P., Zhang Y., Shultz L.D., Prabhu K.S., Paulson R.F, & Zheng H. (2021). A Highly Clinical Relevant Graft-vs-Leukemia Model in Humanized Mice. Journal of leukocyte biology, 111(2), 427-437.

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