Dipalmitoyl sn glycero 3 phosphocholine dppc cholesterol

Manufactured by Avanti Polar Lipids

Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) cholesterol is a lipid compound that consists of a phosphatidylcholine (PC) head group and two palmitoyl fatty acid chains. It is commonly used as a model membrane lipid in various biophysical and biochemical studies.

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Lab products found in correlation

Rpmi 1640 medium, by Cytiva (1 mentions) Alpcs4, by Frontier Scientific (1 mentions) Jc 1 mitochondria staining kit, by Merck Group (1 mentions) Cell counting kit 8, by Dojindo Laboratories (1 mentions) Trypan blue, by Dojindo Laboratories (1 mentions) Sgc 7901, by Cytiva (1 mentions) Ges 1, by Cytiva (1 mentions) Fluo 3am fluorescence probe, by Beyotime (1 mentions) Deadend fluorometric tunel system, by Promega (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Thermo Fisher Scientific (1 mentions) Doxorubicin hcl dox, by LC Laboratories (1 mentions) Gemcitabine gem, by LC Laboratories (1 mentions)

Spelling variants of this product

Cholesterol (209 citations) Dipalmitoyl-sn-glycero-3-phosphocholine (6 citations) 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), cholesterol (4 citations) Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) (3 citations)

2 protocols using dipalmitoyl sn glycero 3 phosphocholine dppc cholesterol

Gastric Cancer Cell Culture Protocol

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Moderately differentiated human gastric cancer cell line SGC-7901 purchased from the Cell Bank of the Academy of Military Medical Science (Beijing, China) and human immortalized fetal gastric epithelial cell line GES-1 obtained from the Beijing Institute of Cancer Research (China)53 (link)–55 (link) were donated by State Key Laboratory of Cancer Biology, the Digestion Department of Xijing Hospital, and the Fourth Military Medical University (China). SGC-7901 and GES-1 cells were cultured in RPMI 1640 medium (HyClone Laboratories Inc., Logan, UT, USA) sup plemented with 10% fetal bovine serum (HyClone) and 1% penicillin/streptomycin in a humidified incubator at 37°C with 5% CO₂. Cetyltrimethylammonium bromide (CTAB), Pluronic F127, and JC-1 mitochondria staining kit were purchased from Sigma. Sodium borohydride, chloroauric acid (HAuCl₄), silver nitrate, and ascorbic acid were purchased from Aladdin, Cell Counting kit (CCK-8) from Dojindo, the trypan blue, Hoechst 33258/propidium iodide (PI) staining kits, Fluo-3/AM fluorescence probe from Beyotime Company, thiol PEG succinimidyl NHS (SH–PEG–NHS) from a local supplier, AlPcS₄ from Frontier Scientific, and dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) cholesterol, and PEG2000-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) from Avanti Polar Lipids.

Xin J., Wang S., Wang B., Wang J., Wang J., Zhang L., Xin B., Shen L., Zhang Z, & Yao C. (2018). AlPcS4-PDT for gastric cancer therapy using gold nanorod, cationic liposome, and Pluronic® F127 nanomicellar drug carriers. International Journal of Nanomedicine, 13, 2017-2036.

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Lipid-based Nanoparticle Drug Delivery

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Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), cholesterol and N-(carbonyl-methoxypolyethylene-glycol-2000)-1, 2-distearoyl-sn-glycero-3-phospho-ethanolamine (DSPE-PEG₂₀₀₀) were purchased from Avanti Polar Lipids (Alabaster, AL). Gemcitabine (Gem) and Doxorubicin HCl (Dox) were purchased from LC laboratories (Woburn, MA). Gemcitabine-5’-triphosphate (2’,2’-difluorodeoxycytidine 5’-triphosphate, dFdCTP) was purchased from Sierra Bioresearch (Tucson, AZ). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Invitrogen (Carlsbad, CA). Terminal deoxynucleotidyl transferase dUTP nick end labeling kit (DeadEnd Fluorometric TUNEL System) was purchased from Promega (Madison, WI). All other materials, including solvents, were purchased from Sigma Aldrich (St. Louis, MO).

Hassan T., Jinho P., Hytham H. G., Masters A.R., Abdel-Aleem J.A., Abdelrahman S.I., Abdelrahman A.A., Lyle L.T, & Yeo Y. (2019). Development of liposomal gemcitabine with high drug loading capacity. Molecular pharmaceutics, 16(7), 2858-2871.

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