The PDS implant was first filled with PBS buffer using an initial fill needle, then refilled using a primed refill needle attached to a 1-mL syringe containing ranibizumab drug product. To assess efficiency of refill-exchange across a range of refill volumes, 20, 40, 60, 80, or 100 µL of ranibizumab 100 mg/mL was delivered accurately by a high-precision syringe pump (Pump 11 Elite Programmable Syringe Pump, Harvard Apparatus, Holliston, MA). After refill, the implant content was collected using an empty 3/10-mL insulin syringe with a 29-gauge (G) needle inserted into the implant septum. The collected sample was analyzed for ranibizumab content using ultraviolet-visible spectrophotometry (UV-VIS spectrophotometer, Model 8453, Agilent Technologies, Santa Clara, CA). Refill efficiency was calculated as the ratio of the ranibizumab concentration measured in the implant after refill vs the concentration of the fill solution.
Pump 11 elite programmable syringe pump
Manufactured by Harvard Apparatus
Sourced in United States
The Pump 11 Elite Programmable Syringe Pump is a precision liquid handling device designed for laboratory applications. It can accurately dispense and withdraw liquids using a variety of syringe sizes. The pump features programmable flow rates, dispense volumes, and other parameters to meet the requirements of various experimental protocols.
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Lab products found in correlation
2 protocols using pump 11 elite programmable syringe pump
1
Refilling Ranibizumab Ocular Implants
2
Microfluidic System for Speckle Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The microfluidics system was made up of a vacuum generator consisting of a syringe pump in withdrawal mode, a solenoid valve, a 3-way valve, and a 50 ml syringe for dead volume. The microfluidic chip contained a channel of 45 mm in length, 1 mm in width, and 45 μm in height. The region of interest (ROI) for the speckle imaging (128 × 512 pixels) was chosen near the outlet reservoir with a 1 mm diameter at the end of the channel sides, as shown in S4 Fig . The treated-blood sample was introduced to the inlet of the microfluidics chip. We used 1 mL syringe racked in the syringe pump (Pump 11 Elite Programmable Syringe Pump, Harvard Apparatus, US), the pulling volume was fixed at 200 μL using a withrawal flow rate of 5 mL/min. When the solenoid valve was opened, the treated-blood samples were driven by a negative pressure gradient inside the microchamber. We applied a relatively large dead volume chamber, which was connected to a syringe pump to relieve pressure fluctuation during the experiment. The treated blood began to flow into the microfluidics channel under a constant pressure gradient.
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