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Dtx880 plate reader

Manufactured by BD
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The DTX880 plate reader is a multi-mode microplate reader designed for a wide range of applications. It can perform absorbance, fluorescence, and luminescence measurements. The device is capable of detecting a variety of sample types in 6- to 384-well microplates.

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Lab products found in correlation

Human specific elisa kit, by BD (2 mentions)

2 protocols using dtx880 plate reader

1

TRIM9 Regulates Cytokine Production

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For overexpression, HEK293T cells were transfected for 24 h with TRIM9 WT, TRIM9 SA or vector. Cells were then stimulated for 24hrs with IL-1 β, TNF-α or PMA/Ionomycin. SK-N-AS cells stably transfected with TRIM9 WT, SA mutant or vector were stimulated with IL-1 β for 24hrs. For knockdown assay, SK-N-AS and A549 stable knockdown TRIM9 or control cells were stimulated with IL-1 β, TNF-α or PMA/Ionomycin respectively. Supernatants were collected and their concentration of IL-6 or TNF-α was determined with a human-specific ELISA kit (BD Biosciences), followed by analysis with a Beckman Coulter DTX880 plate reader.
Shi M., Cho H., Inn K.S., Yang A., Zhao Z., Liang Q., Versteeg G.A., Amini-Bavil-Olyaee S., Wong L.Y., Zlokovic B.V., Park H.S., García-Sastre A, & Jung J. (2014). Negative regulation of NF-κB activity by brain-specific TRIpartite Motif protein 9. Nature communications, 5, 4820.
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2

TRIM9 Regulates Cytokine Production

Check if the same lab product or an alternative is used in the 5 most similar protocols
For overexpression, HEK293T cells were transfected for 24 h with TRIM9 WT, TRIM9 SA or vector. Cells were then stimulated for 24hrs with IL-1 β, TNF-α or PMA/Ionomycin. SK-N-AS cells stably transfected with TRIM9 WT, SA mutant or vector were stimulated with IL-1 β for 24hrs. For knockdown assay, SK-N-AS and A549 stable knockdown TRIM9 or control cells were stimulated with IL-1 β, TNF-α or PMA/Ionomycin respectively. Supernatants were collected and their concentration of IL-6 or TNF-α was determined with a human-specific ELISA kit (BD Biosciences), followed by analysis with a Beckman Coulter DTX880 plate reader.
Shi M., Cho H., Inn K.S., Yang A., Zhao Z., Liang Q., Versteeg G.A., Amini-Bavil-Olyaee S., Wong L.Y., Zlokovic B.V., Park H.S., García-Sastre A, & Jung J. (2014). Negative regulation of NF-κB activity by brain-specific TRIpartite Motif protein 9. Nature communications, 5, 4820.
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