Psv β galactosidase vector and luciferase assay kit

Manufactured by Promega

Sourced in United States

The PSV-β-galactosidase vector and luciferase assay kit are laboratory tools used for gene expression analysis. The PSV-β-galactosidase vector is a plasmid that contains the β-galactosidase reporter gene. The luciferase assay kit is used to measure the activity of the luciferase enzyme, which is commonly used as a reporter for gene expression studies. These products provide researchers with the necessary tools to investigate and quantify gene expression in various experimental systems.

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PSV-β-galactosidase vector PSV-β-galactosidase β-galactosidase assay kit β-galactosidase Luciferase assay Promega luciferase kits

2 protocols using psv β galactosidase vector and luciferase assay kit

Adiponectin Signaling Pathway Analysis

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Rabbit polyclonal antibodies specific for ICAM-1, p-AMPK, AMPK, p-LKB1, LKB1, p-CaMKII, CaMKII, p-c-Jun, c-Jun, and β-actin, anti-mouse and anti-rabbit IgG-conjugated horseradish peroxidase, and Protein A/G beads were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Compound C and adenosine-9-β-d-arabino-furanoside (AraA) were purchased from Calbiochem (San Diego, CA). Human full-length adiponectin was purchased from R&D Systems (Minneapolis, MN). The AP-1 luciferase plasmid was purchased from Stratagene (La Jolla, CA). The pSV-β-galactosidase vector and luciferase assay kit were purchased from Promega (Madison, MA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).

Chen H.T., Tsou H.K., Chen J.C., Shih J.M., Chen Y.J, & Tang C.H. (2014). Adiponectin Enhances Intercellular Adhesion Molecule-1 Expression and Promotes Monocyte Adhesion in Human Synovial Fibroblasts. PLoS ONE, 9(3), e92741.

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Adiponectin Signaling Pathway Characterization

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Anti-mouse and anti-rabbit horseradish peroxidase-conjugated IgG, rabbit polyclonal antibodies against AdipoR1, AdipoR2, β-actin, phosphorylated-p85 (p-p85), p85, phosphorylated-Akt (p-Akt), Akt, phosphorylated-mTOR (p-mTOR), and mTOR were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HIF-1α, VEGF-A, and CD31 antibodies were purchased from Abcam (Cambridge, MA, USA). Recombinant human adiponectin and VEGF-A were purchased from R&D Systems (Minneapolis, MN, USA). pHRE-luciferase construct was provided from Dr. W.M. Fu (National Taiwan University, Taiwan). The pSV-β-galactosidase vector and luciferase assay kit were purchased from Promega (Madison, WI, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Lee H.P., Lin C.Y., Shih J.S., Fong Y.C., Wang S.W., Li T.M, & Tang C.H. (2015). Adiponectin promotes VEGF-A-dependent angiogenesis in human chondrosarcoma through PI3K, Akt, mTOR, and HIF-α pathway. Oncotarget, 6(34), 36746-36761.

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