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Uplansapo 60 1.35 oil objective lens

Manufactured by Olympus
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The UPlanSApo 60× /1.35 oil objective lens is a high-performance microscope objective designed for use in various scientific applications. It features a numerical aperture of 1.35 and a 60× magnification, providing high-resolution imaging capabilities. The objective is suitable for use with oil immersion techniques.

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Lab products found in correlation

Fluoview 1000, by Olympus (2 mentions) Hepes buffer, by Merck Group (2 mentions) Ix81 confocal microscope, by Olympus (2 mentions) Phenol red free dmem, by Merck Group (2 mentions) 37 c heating stage, by Ibidi (2 mentions)

Close spelling variants of this product

UPlanSApo (172 citations) 100× objective lens (88 citations) UPlanSApo objective (31 citations) UPlanSApo 60× 1.35 (9 citations) UPlanSApo 60× (9 citations) UPlanSApo 60 /1.35 Oil (2 citations) UPlanSApo 60× Oil (2 citations) 60x/1.42 oil objectives (2 citations) UPlanSApo × 60/1.35 oil immersion objective lens (2 citations)

2 protocols using uplansapo 60 1.35 oil objective lens

1

UV-A Laser-Induced DNA Damage Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
U2OS cells stably expressing RFP-53BP1 in 35 mm glass-bottom dishes (WillCo-dish) were transfected with 1 μg of expression plasmids coding each GFP-DUB with FuGENE 6 and further cultured for 48 hours in the presence of 10 μM 5-Bromo-2′-deoxyuridine (BrdU). On the day of analysis, the media was replaced with phenol red-free DMEM (Sigma-Aldrich) supplemented with 10% FBS, penicillin, streptomycin and 25 mM HEPES buffer (pH 7.0-7.6, Sigma-Aldrich). DNA damage was induced by irradiating cells through a UPlanSApo 60 × /1.35 oil objective lens with UV-A laser (405 nm) using a IX81 confocal microscope (Olympus) equipped with a 37°C heating stage (Ibidi). The laser output was set at 400 μW with 50 scans of 10 msec/pixel. Up to 1 hour after damage induction, images were taken and analyzed by using FluoView 1000 software (Olympus).
Nishi R., Wijnhoven P., le Sage C., Tjeertes J., Galanty Y., Forment J.V., Clague M.J., Urbé S, & Jackson S.P. (2014). Systematic characterization of deubiquitylating enzymes for roles in maintaining genome integrity. Nature cell biology, 16(10), 1016-8.
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2

UV-A Laser-Induced DNA Damage Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
U2OS cells stably expressing RFP-53BP1 in 35 mm glass-bottom dishes (WillCo-dish) were transfected with 1 μg of expression plasmids coding each GFP-DUB with FuGENE 6 and further cultured for 48 hours in the presence of 10 μM 5-Bromo-2′-deoxyuridine (BrdU). On the day of analysis, the media was replaced with phenol red-free DMEM (Sigma-Aldrich) supplemented with 10% FBS, penicillin, streptomycin and 25 mM HEPES buffer (pH 7.0-7.6, Sigma-Aldrich). DNA damage was induced by irradiating cells through a UPlanSApo 60 × /1.35 oil objective lens with UV-A laser (405 nm) using a IX81 confocal microscope (Olympus) equipped with a 37°C heating stage (Ibidi). The laser output was set at 400 μW with 50 scans of 10 msec/pixel. Up to 1 hour after damage induction, images were taken and analyzed by using FluoView 1000 software (Olympus).
Nishi R., Wijnhoven P., le Sage C., Tjeertes J., Galanty Y., Forment J.V., Clague M.J., Urbé S, & Jackson S.P. (2014). Systematic characterization of deubiquitylating enzymes for roles in maintaining genome integrity. Nature cell biology, 16(10), 1016-8.
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