Mouse adiponectin elisa kit ezmadp 60k

Plasma levels of lipid metabolites such as triglycerides (TG), non-esterified fatty acids (NEFA) and total cholesterol, and enzymes aspartate transaminase (AST) and alanine aminotransferase (ALT) were measured using the assays from Roche or Wako (for NEFA) and a Clinical Chemistry analyzer Roche/Hitachi 902 (Roche Diagnostics; Basel, Switzerland). Plasma levels of insulin and the levels of leptin and C-C motif chemokine ligand 2 (CCL2) in epididymal WAT were quantified using xMAP technology and MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel (MMHMAG-44K; Merck-Millipore). Plasma levels of total adiponectin were measured by Mouse Adiponectin ELISA kit (EZMADP-60K; Sigma-Aldrich). Fasting plasma insulin and blood glucose levels were used to quantify Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), using the formula: plasma insulin (mU/L) x blood glucose (mmol/L)/22.5.

Plasma levels of lipid metabolites (i.e., TAGs, total cholesterol, non-esterified fatty acids), as well as aspartate transaminase (AST) and alanine aminotransferase (ALT), were measured using the appropriate assays from Roche or Wako (for the measurement of non-esterified fatty acids) and a Clinical Chemistry analyzer Roche/Hitachi 902 (Roche Diagnostics; Basel, Switzerland). Plasma levels of insulin were quantified using xMAP technology and MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel (MMHMAG-44K; Merck-Millipore; Burlington, MA, USA). Plasma levels of total adiponectin were measured by Mouse Adiponectin ELISA kit (EZMADP-60K; Sigma-Aldrich). Fasting plasma insulin and blood glucose levels were used to quantify Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), using the following formula: fasting plasma insulin (mU/L) × fasting plasma glucose (mmol/L)/22.5.