Epigallocatechin gallate (egcg)

RKO cells were purchased from Procell (Wuhan, China), and HCT116 cells were purchased from NuoHe Bio-Tech (Chengdu, China). All the cell lines used have been authenticated, and mycoplasma testing has been carried out. EGCG, irinotecan, and JC-1 were purchased from CSN pharm (Shanghai, China), Bradford protein concentration assay kit (detergent-compatible type), and reactive oxygen species detection kit were purchased from Beyotime (Shanghai, China). GAPDH, ACTIN, Bax, Bcl-2, PARP, GRP78, Ki67, and cleaved-caspase3 antibodies were purchased from Proteintech (Wuhan, China). The GRP78 overexpression plasmid was designed by Genechem (Shanghai, China), and the GRP78 siRNA was provided by Tsingke (Beijing, China). Lipofectamine™ 2000 Transfection Reagent was purchased from Invitrogen (California, USA). Trizol reagent and HiScript® III reverse transcription kit was purchased from Vazyme (Nanjing, China). FastQuant RT Kit (With gDNase) reverse transcription kit was purchased from Tiangen (Beijing, China), and TB Green Premix Ex Taq II (Tli RNaseH Plus) was purchased from Takara (Dalian, China). Annexin V-FITC apoptosis assay kit was purchased from Absin (Shanghai, China). SABC (mouse IgG)-POD kit was provided by Solarbio (Beijing, China).

EGCG and irinotecan were purchased from CSN Pharm (Shanghai, China), Dansylcadaverine (MDC) from Sigma‐Aldrich and Cell Counting Kit‐8 (CCK8) from Bimake. DNA Damage Antibody Sampler Kit, CDK4, LC3B, Cyclin D 1 and P‐RBSER807/811 were purchased from CST and Top1, GAPDH, BAX, BCL‐2, PARP, Beclin‐1 and P62 from Proteintech Group. Goat anti‐Rabbit IgG‐FITC antibody was purchased from Hua'an. Annexin V‐FITC Apoptosis assay kit was purchased from Vazyme. A cell cycle and apoptosis assay kit was purchased from Beyotime Biotechnology.