Estrone 3 o sulfamate

Manufactured by Merck Group

Estrone-3-O-sulfamate is a chemical compound used in laboratory settings. It functions as a steroid hormone precursor. The compound is utilized in various research and analytical applications where the study of steroid hormones is required.

Automatically generated - may contain errors

Lab products found in correlation

Nonidet p 40, by Roche (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions)

2 protocols using estrone 3 o sulfamate

Quantifying Cellular Steroid Sulfatase Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five days after treatment with 5-Aza-dC, SNU449 and Huh7 cells were washed in ice-cold PBS and lysed in SIE buffer (250 mM sucrose, 3 mM imidazole, pH 7.4, 1% ethanol) containing 1% (w/v) Nonidet P-40 and protease inhibitor cocktail (Roche Molecular Biochemicals). A total cellular protein of 100 μg was preincubated with 10 μM estrone-3-O-sulfamate (Sigma) at 37°C for 1 h to inhibit steroid sulfatases. 4-Methylumbelliferyl sulfate (4-MUS) was then added at 10 mM in 10 mM lead acetate in a 200-μl volume. After incubation for 24 h at 37°C, the reaction was terminated using 1 ml 0.5 M NaHCO₃, pH 10.7. Fluorescence of the liberated 4-methylumbelliferone was measured using excitation and emission wavelengths of 365 and 450 nm, respectively.

Shire A., Lomberk G., Lai J.P., Zou H., Tsuchiya N., Aderca I., Moser C.D., Gulaid K.H., Oseini A., Hu C., Warsame O., Jenkins R.B, & Roberts L.R. (2015). Restoration of Epigenetically Silenced Sulfatase 1 Expression by 5-Aza-2′-Deoxycytidine Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy-Induced Apoptosis. Medical Epigenetics, 3(1), 1-18.

+ Open protocol

+ Expand

Cellular Steroid Sulfatase Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five days after treatment with 5-Aza-dC, SNU449 and Huh7 cells were washed in ice-cold PBS and lysed in SIE buffer (250 mM sucrose, 3 mM imidazole, pH 7.4, 1% ethanol) containing 1% (w/v) Nonidet P-40 and protease inhibitor cocktail (Roche Molecular Biochemicals). A total cellular protein of 100 µg was preincubated with 10 µM estrone-3-O-sulfamate (Sigma) at 37°C for 1 h to inhibit steroid sulfatases. 4-methylumbelliferyl sulfate (4-MUS) was then added at 10 mM in 10 mM lead acetate in 200 µl volume. After incubation for 24 h at 37°C, the reaction was terminated using 1 ml 0.5 M NaHCO₃, pH 10.7. Fluorescence of the liberated 4-methylumbelliferone was measured using excitation and emission wavelengths of 365 and 450 nm, respectively.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!