Jem 1400plus system

Manufactured by JEOL

Sourced in Japan

The JEM-1400plus is a transmission electron microscope (TEM) system designed for high-resolution imaging and analysis of a wide range of materials. It features a LaB6 electron source and a robust column design to provide stable and reliable performance. The JEM-1400plus system enables users to obtain detailed structural and compositional information about their samples at the nanoscale level.

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Lab products found in correlation

Zetasizer nano zsp instrument, by Malvern Panalytical (4 mentions) Lsm 700, by Zeiss (2 mentions) N a 1.4 oil, by Zeiss (1 mentions)

6 protocols using jem 1400plus system

Characterizing TTCI Nanoparticle Size and Morphology

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A dynamic light scattering (DLS) Zetasizer nano zsp instrument (Malvern instruments Led) was used to measure the diameter distribution at room temperature. The size of TTCI NPs with different proportions was first characterized. 200 µL TTCI NPs (different proportions, 1mM) were diluted with deionized water to produce 1 mL aqueous solution. The diameter distribution of the diluted aqueous solution was measured at room temperature.
The morphology of TTCI NPs (TTCI:DSPE-PEG2000 = 1:0.2 (mol:mol)) was observed by transmission electron microscopy (TEM) on a JEM-1400plus system (JEOL, Japan); 30 µL TTCI NPs (1 mM) was diluted to 100 µL aqueous solution and mixed together. The diluted solution was applied to a copper grid and 0.2% (w/v) phosphotungstic acid aqueous solution was used to stain the samples.

Zhang Y.M., Xia M., Ao R., Gao L.X., Tang Y., Huang J.H., Luo Y.F., Chen Z.Z., Wang B.C, & Huang Z. (2021). Smart Design of Mitochondria-Targeted and ROS-Responsive CPI-613 Delivery Nanoplatform for Bioenergetic Pancreatic Cancer Therapy. Nanomaterials, 11(11), 2875.

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Characterizing Nanoparticles via DLS and TEM

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The size of NPs with different proportions was first characterized by dynamic light scattering (DLS) using Zetasizer Nano ZSP instrument (Malvern Instruments Ltd) at room temperature. Then, the size and morphology of Palb-TK-Palb NPs and Palb-TK-Palb/Ce6 NPs were measured by DLS and TEM (JEM-1400plus system (JEOL, Japan)).
The storage stability of Palb-TK-Palb/Ce6 NPs was examined. After storing at 4 °C for 1, 5 and 12 days, the particle size was examined by DLS. The Palb-TK-Palb/Ce6 NPs were incubated under 100 mM H₂O₂ or PBS (0.05 M, pH 5.0) at 37 °C for 48 h. The changes in size were monitored by DLS.

Huang Z., Hu H., Xian T., Xu Z., Tang D., Wang B, & Zhang Y. (2023). Carrier-free nanomedicines self-assembled from palbociclib dimers and Ce6 for enhanced combined chemo-photodynamic therapy of breast cancer. RSC Advances, 13(3), 1617-1626.

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Nanoparticle Characterization and Stability

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The diameter distribution and zeta potential were measured by the dynamic light scattering (DLS) Zetasizer nano zsp instrument (Malvern instruments Led). The storage stability was assayed by keeping it at 4 degrees for several days and serum stability was assayed after serum incubation, respectively. The morphology was observed by a JEM-1400 plus system (JEOL, Kyoto, Japan). The drug loading content was determined through UV–Vis spectra and HRMS.
To investigate the assembly mechanism, the UV–Vis spectra of NP-0% was measured after incubation in sodium dodecyl sulfate (SDS) or urea solution.

Huang Z., Xian T., Meng X., Hu H., Gao L., Huang J., Yang D., Ou K., Wang B, & Zhang Y. (2023). Multifunctional Novel Nanoplatform for Effective Synergistic Chemo-Photodynamic Therapy of Breast Cancer by Enhancing DNA Damage and Disruptions of Its Reparation. Molecules, 28(19), 6972.

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Ultrastructural Analysis of Regenerated Nerve Myelin

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After gently removing the OCT, the regenerated nerves excised from rats were fixed in a mixture of 2.5% glutaraldehyde and 2% paraformaldehyde dissolved in PBS for 1 h. After postfixing with 1% osmium tetroxide in PBS for 1 h, the samples were dehydrated in a gradient concentration of ethanol ranging from 50% to 100% and then infiltrated and embedded in pure LR White (medium grade, London Resin Co., Ltd., Berkshire, England). Ultrathin sections of the regenerated nerve at the central region were counterstained with uranyl acetate and lead citrate. Transmission electron microscopy (TEM) images were taken using JEM-1400 Plus system (JEOL Ltd.) to measure the myelin sheath thickness (μm). Ten TEM images were selected from the central region of the regenerated myelin sheath in the OCT group and intact group, and the myelin sheath thickness was calculated from the defined field (35 μm × 26 μm) of the cross section; their average values were compared.

Otake K., Toriumi T., Ito T., Okuwa Y., Moriguchi K., Tanaka S., Isobe Y., Saku T., Kurita K, & Honda M. (2020). Recovery of sensory function after the implantation of oriented-collagen tube into the resected rat sciatic nerve. Regenerative Therapy, 14, 48-58.

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Nanoparticle Characterization and Stability

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Multimodal Imaging of Neuronal Structures

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Slices or cells were imaged under an inverted laser scanning confocal microscope by either (LSM-700, Zeiss) or (SP5, Leica) with a 63x objective (N.A 0.7, LEICA) at a resolution of 2048x2048 (pixel size 0.246 mm) and a 100x objective (N.A 1.4 oil, Zeiss) respectively. Images acquisition for the GNPs was performed using a 3D microscope (Optigrid, Leica) with a 63x objective (N.A 1.40, LEICA) connected to a CCD camera (pixel size 6.5 mm x 6.5 mm). The excitation wavelengths were 405 nm for DAPI, 488 nm for EGFP, 555 nm for red fluorescence, and 635 nm for infrared. Zeiss LSM 7MP with 20X (N.A. 1.0) was used to observe the granule neurons with T-shape morphology. A femtosecond pulsed infrared laser (MaiTai HP, SpectraPhysics) tuned at 910 nm was used as the excitation light source.
Electron Microscopy P6 mice pups were transcardially fixed with a buffer containing 2.5% glutaraldehyde, 4% PFA in 0.1M PBS. Next, cerebella were processed and sectioned at the Transmission Electron Microscope Facility in Academia Sinica as previously described (Gray, 1961) . Cerebella sections were observed under JEOL JEM-1400 PLUS system.

Chang C.H., Zanini M., Shirvani H., Cheng J.S., Yu H., Feng C.H., Mercier A.L., Hung S.Y., Forget A., Wang C.H., Cigna S.M., Lu I.L., Chen W.Y., Leboucher S., Wang W.J., Ruat M., Spassky N., Tsai J.W, & Ayrault O. (2019). Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation. Developmental cell, 48(2).

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