Plasma samples were taken at baseline, after 30 min and every 60 min after LPS installation. BALF samples were obtained at baseline and the end with an Ambu® aScopeTM (Ambu). Plasma and BALF were analyzed with the cytokine multiplex kit Cytokine & Chemokine 9‐Plex Porcine ProcartaPlex™ Panel 1 (Thermo Fisher Scientific Cat. No. EPX090‐60829–901) according to the manufacturer's instructions. The kit was analyzed using a Bioplex‐200 system (BioRad). The six cytokines in the kit included: IL‐1β, IL‐6, IL‐8, IL‐10, IL‐12, and TNF‐α.
Ascope
Manufactured by Ambu
Sourced in Denmark
The Ambu® aScopeTM is a portable, single-use video laryngoscope designed for intubation procedures. It provides a clear, high-quality video display to assist healthcare professionals in visualizing and accessing a patient's airway.
Automatically generated - may contain errors
6 protocols using ascope
1
Cytokine Profiling in Porcine LPS-Induced Lung Injury
2
Postmortem Transbronchial Cryobiopsy for Lung Sampling
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Postmortem transbronchial cryobiopsy samples were obtained as previously described [11 (link)]. After patient death, patient lung aeration was maintained by applying a constant positive airway pressure equal to the positive end-expiratory pressure received before death. As per national regulations, death was ascertained with a continuous 20-min flat electrocardiogram, while the sampling procedure was initiated within 30 min from death. Cryobiopsies were obtained with a 1.7 mm cryoprobe (ErbeCryo®, Erbe Elektromedizin GmbH, Tuebingen, Germany) inserted through the operative channel of a single use flexible video-bronchoscope (Ambu® aScopeTM, Ambu, Ballerup, Denmark) and operated for 10–11 s. Biopsies were collected and immediately fixed in 10% formalin for each lobe: right lower lobe (RLL), right middle lobe (RML), right upper lobe (RUL), left lower lobe (LLL) and left upper lobe (LUL).
3
Post-Mortem Transbronchial Lung Cryobiopsy
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The methodology described for pmTBLC is slightly different compared with in vivo TBLC; though perhaps inappropriate, the term cryobiopsy (and not cryosample as would be more fitting in the deceased) is used throughout as it refers more to the procedure than the sample itself. All patients were already intubated and mechanically ventilated with an endotracheal tube. At death, heparin infusion was immediately stopped, and subsequently a single-use, flexible video-bronchoscope (Ambu®aScope™, Ambu A/S, Ballerup, Denmark) with a 2.6-mm-wide operative channel was introduced. A 1.7-mm-wide cryoprobe (ErbeCryo®, Erbe Elektromedizin GmbH, Tuebingen, Germany) was inserted within the working channel, and the cryoprobe was then frozen for approximately 10–11 s and biopsy performed.
The sampling protocol included 2 biopsies at each site starting from the right lower lobe (RLL) and continuing to the right middle lobe (RML), right upper lobe (RUL), left lower lobe (LLL) and left upper lobe (LUL). A maximum of 10 biopsies were collected and immediately fixed in 10% buffered formalin.
During all procedures, operators wore full protective equipment including whole body suits, protective hoods, moulded protection masks (FFP3), visors, goggles and double-layer gloves. No operator infection occurred.
The sampling protocol included 2 biopsies at each site starting from the right lower lobe (RLL) and continuing to the right middle lobe (RML), right upper lobe (RUL), left lower lobe (LLL) and left upper lobe (LUL). A maximum of 10 biopsies were collected and immediately fixed in 10% buffered formalin.
During all procedures, operators wore full protective equipment including whole body suits, protective hoods, moulded protection masks (FFP3), visors, goggles and double-layer gloves. No operator infection occurred.
4
Acid-induced Lung Injury Model
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Lung injury was accomplished by endobronchial administration of acid. HCl 0.05 N, pH 1.41, was prepared and instilled (8 ml/kg body weight) at the right cranial lobe bronchus, the right main bronchus, and the left main bronchus, in the ratio of 1:2:3 over 3 min by means of a flexible bronchoscope (Ambu®ascope™). We instilled the acid directly after intubation and allowed 60 min post instillation for lung injury to become established. In uninjured animals, bronchoscopy was performed at identical timepoints, but without any instillation of acid or vehicle.
5
Videobronchoscopy in COVID-19 Patients
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In this work Authors report the experience acquired on "WHEN, WHY and HOW" 87 videobronchoscopies were performed in patients with suspected or confirmed COVID-19, from 03/01/2020 to 06/01/2020 using a single-use bronchoscope (Ambu ® aScope ™ ) and with a bronchoscopy setting for COVID-19 patients. All procedures were performed in a single center "Azienda Ospedaliera dei Colli", Cotugno and Monaldi hospitals by a multidisciplinary team made up of pulmonologists and anesthetists and under the supervision of infectious disease specialists. During the most dramatic phase of the novel coronavirus health crisis in Italy, 79 videobronchoscopies have been performed in confirmed COVID-19 patients and 8 videobronchoscopies in patients with clinical and radiological suspect of COVID-19. The informed consent collection took place in all non-intubated patients before the procedure while in intubated patients the procedure was performed urgently.
6
Airway Management with High-Fidelity Simulator
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The airway of an adult high-fidelity simulator (SimMan™ 3G; Laerdal® Medical, Stavanger, Norway) was manipulated according to the needs of the particular scenario by the manufacturer’s settings for tongue oedema and pharyngeal obstruction, whereas adequate chest excursion and breath sounds were adjusted according to the scenario. The departmental anaesthesia ventilator (Pallas, Dräger®, Lübeck, Germany), standardised airway, and anaesthesia trolley were used. The airway trolley included the following devices: (i) one type of videolaryngoscope (VL) C-MAC® Macintosh blade, sizes 3 and 4, or hyperangulated blade (Karl Storz®, Tuttlingen, Germany), or standard laryngoscope, with a Macintosh blade, sizes 3 and 4 (DL; Heine® Optotechnik, Gilching, Germany); (ii) a supraglottic airway device (SGA): Ambu® Aura Gain®, sizes 4 and 5 (Ambu® A/S, Copenhagen, Denmark); (iii) different airway exchange catheters (AECs): Cook® Airway Exchange Catheter (Cook® Medical, Bloomington, IN, USA), S-Guide™, or Muallem Stylet™ (VBM®, Sulz a. N., Germany), and Aintree Intubation Catheter™ (Cook® Medical, Bloomington, IN, USA); and (iv) a flexible (video) endoscope (FO): aScope®, sizes slim to large (Ambu® A/S, Copenhagen, Denmark).
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