The antibodies used in western blot were shown as follows: ADARB1 (22248-1-AP, Proteintech), α-Tubulin (sc-69969, Santa Cruz). Each protein sample, forty micrograms, was isolated by 8% SDS-PAGE. Then, the samples were transferred to the surface of polyvinylidene fluoride membrane and probed with the appropriate primary antibodies (SA00001-1, Goat anti-mouse IgG (H+L) HRP conjugate, Proteintech; SA00001-2, Goat anti-rabbit IgG (H+L) HRP conjugate, Proteintech) afterwards. The protein bands were visualized by immobilon western chemiluminescent reagents (WBKLS0500, Millipore).
Goat anti mouse igg h l hrp conjugate
Manufactured by Proteintech
Goat anti-mouse IgG (H+L) HRP conjugate is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to mouse immunoglobulin G (IgG) antibodies, recognizing both the heavy (H) and light (L) chains.
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Lab products found in correlation
2 protocols using goat anti mouse igg h l hrp conjugate
1
Western Blot Quantification Protocol
2
Western Blot Analysis of Cellular Proteins
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Cells and tumor tissues were washed with ice-cold PBS and lysed with RIPA buffer containing protease inhibitors. Proteins were quantified using a BCA kit after centrifugation at 4 °C, separated by SDS-PAGE, and transferred to polyvinylidene difluoride membranes (pore size, 0.22 μm, Millipore, USA). Membranes were blocked with 5% skim milk and incubated with primary antibodies overnight at 4°C. The antibodies used were as follows: RACGAP1 (Proteintech, cat no. 13739-1-AP and Abcam, cat. no. ab97315), GAPDH (Proteintech, cat no. 60004-1-Ig and Abcam, cat. no. ab8245); MCM3 (Proteintech, cat no. 15597-1-AP); YY1 (Proteintech, cat no. 66,281–1-Ig), goat anti-mouse IgG (H + L)/HRP conjugate (Proteintech, cat no. SA00001-1), and goat anti-rabbit IgG (H + L)/HRP conjugate(Proteintech, cat. no. SA00001–2). Chemiluminescent imaging was performed using the Tanon 5200 system in conjunction with the SuperSignal West Pico Plus reagent kit (Thermo Fisher Scientific, Waltham, MA, USA), and the target proteins were analyzed by ImageJ software.
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