2 2 azobis 2 amidinopropane dihydrochloride solution abap

Dimethyl sulfoxide (DMSO), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox, fluorescein sodium salt, 2′,7′-dichlorfluorescin diacetate (DCFH–DA), and 2,2-azobis (2-amidinopropane) dihydrochloride solution (ABAP) were purchased from Sigma Chemical Co. (Sigma-Aldrich, St. Louis, MO, USA). Flavonoid standards were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Phosphate buffer (PBS), MEM/EBSS, foetal bovine serum (FBS), penicillin, and streptomycin were purchased from HyClone (Logan, UT, USA). Cell Counting Kit-8 was obtained from Dojindo China Co., Ltd. (Shanghai, China). Kits for the determination of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were purchased from Beyotime Biotechnology (Shanghai, China).

Dimethyl sulfoxide (DMSO), 2′,7′-dichlorfluorescin diacetate (DCFH–DA), and 2,2-azobis (2-amidinopropane) dihydrochloride solution (ABAP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Epigallocatechin gallate (EGCG, HPLC grade, purity > 98%) and kaempferol (HPLC grade, purity > 98%) were obtained from Solarbio Science & Technology Co., Ltd. (Beijing, China). EGCG and kaempferol were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mg/mL and then freshly diluted in culture medium. The final concentration of DMSO in culture medium was below 0.05% (v/v). Phosphate buffer solution (PBS), minimum Eagle’s medium (MEM), fetal bovine serum (FBS), 0.05% trypsin–EDTA solution, penicillin (10,000 u/mL), and streptomycin (10,000 μg/mL) were purchased from HyClone (Logan, UT, USA). The Cell Counting Kit-8 (CCK-8) was obtained from Dojindo China Co., Ltd. (Shanghai, China). The BCA protein concentration test kit; cell lysis buffer for Western and IP; and phenylmethanesulfonyl fluoride (PMSF, 100 mM), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) assay kits were purchased from Beyotime Biotechnology (Shanghai, China). All other chemicals and reagents with analytical grade used in this study were obtained from Sinopharm Group Chemical Reagents Co. Ltd. (Beijing, China).