The lysate from Hep3B and Huh7 cells was prepared using RIPA lysis buffer (radio-immunoprecipitation assay buffer), and the protein concentration was measured with Pierce™ BCA Protein Assay Kit (23,225, ThermoFisher, American). The level of SOX2 was detected with the anti-SOX2 antibody (2748, Cell signaling, American). HIF1A antibody (AF1935, R&D Systems, America), cleaved caspase3 antibody (8202S, Cell Signaling Technology, America) and PCNA antibody (GTX100539, GeneTex, China) were used in this study.
Hif1a antibody
Manufactured by R&D Systems
The HIF1A antibody is a laboratory tool used to detect and measure the presence of the HIF1A protein in biological samples. HIF1A is a transcription factor that plays a key role in the cellular response to hypoxia. This antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and ELISA, to study the expression and regulation of HIF1A in different contexts.
Automatically generated - may contain errors
3 protocols using hif1a antibody
1
Protein Expression Analysis in Liver Cancer Cells
2
Protein Expression Analysis in Liver Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The lysate from Hep3B and Huh7 cells was prepared using RIPA lysis buffer (radio-immunoprecipitation assay buffer), and the protein concentration was measured with Pierce™ BCA Protein Assay Kit (23225, ThermoFisher, American). The level of SOX2 was detected with the anti-SOX2 antibody (2748, Cell signaling, American). HIF1A antibody (AF1935, R&D Systems, America), cleaved caspase3 antibody (8202S, Cell Signaling Technology, America) and PCNA antibody (GTX100539, GeneTex, China) were used in this study.
3
Protein Expression Analysis in Liver Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The lysate from Hep3B and Huh7 cells were prepared using RIPA lysis buffer (radio-immunoprecipitation assay buffer), and the protein concentration was measured with Pierce™ BCA Protein Assay Kit (23225, ThermoFisher, American). Finally, the level of SOX2 was detected with the anti-SOX2 antibody (2748, Cell signaling, American). HIF1A antibody (AF1935, R&D Systems, America), cleaved caspase3 antibody (8202S, Cell Signaling Technology, America) and PCNA antibody (GTX100539, GeneTex, China) were used in this study.
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