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Ki67 and keratin 6

Manufactured by Leica

Ki67 and keratin 6 are laboratory markers used for research purposes. Ki67 is a protein expressed during active phases of the cell cycle, while keratin 6 is a structural protein found in certain epithelial cells. These markers can be used to study cell proliferation and tissue composition, but their specific applications and intended uses are not being described here.

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2 protocols using ki67 and keratin 6

1

Immunostaining Protocol for Cellular Markers

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Example 8

Reagents. Antibodies used: Ki67 and keratin 6 (NovoCastra); PCNA (PC10) and αSMA (alpha-SM1) (Santa Cruz Biotechnology); CD31 (BD Pharmingen); CD68 (FA-11) (Biolegend); F4/80 (AbD Serotec); cANGPTL4: monoclonal antibodies against the C-terminal mouse (190-410 amino acids) of ANGPTL4 were produced by ProSci, respectively; goat anti-rabbit and anti-mouse IgG-HRP (Santa Cruz Biotechnology); Alexa Fluor 488 or 594 goat anti-mouse IgG, anti-rat IgG and anti-rabbit IgG (Molecular probes). DAB peroxidase substrate kit (Vector Laboratories). Unless mentioned otherwise, all chemicals were from Sigma-Aldrich and molecular biology enzymes from Fermentas. All oligonucleotides were synthesized by Sigma-Proligo.

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2

Antibody Panel for Immunohistochemistry

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Example 8

Reagents.

Antibodies used: Ki67 and keratin 6 (NovoCastra); PCNA (PC10) and αSMA (alpha-SM1) (Santa Cruz Biotechnology); CD31 (BD Pharmingen); CD68 (FA-11) (Biolegend); F4/80 (AbD Serotec); cANGPTL4: monoclonal antibodies against the C-terminal mouse (190-410 amino acids) of ANGPTL4 were produced by ProSci, respectively; goat anti-rabbit and anti-mouse IgG-HRP (Santa Cruz Biotechnology); Alexa Fluor 488 or 594 goat anti-mouse IgG, anti-rat IgG and anti-rabbit IgG (Molecular probes). DAB peroxidase substrate kit (Vector Laboratories). Unless mentioned otherwise, all chemicals were from Sigma-Aldrich and molecular biology enzymes from Fermentas. All oligonucleotides were synthesized by Sigma-Proligo.

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