Briefly, 5 × 104–2 × 105/mL H9c2 cells were planted on a 96-well plate and incubated at 37°C for 6 hours, followed by adding the LDH solution (Mlbio, Shanghai, China) and incubating for another 1 hour. Lastly, the microplate reader (Mindray, Shenzhen, China) was used to measure the absorbance at 492 nm to determine the release of LDH [16 (link)].
Ldh solution
Manufactured by Mlbio
Sourced in China
LDH solution is a laboratory reagent used for the quantitative determination of lactate dehydrogenase (LDH) activity in biological samples. It is a clear, colorless liquid that serves as a source of the enzyme LDH, which is essential for various metabolic processes in cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ldh solution
1
LDH Assay for Cell Viability
2
LDH Release Assay in 96-well Plate
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In brief, cells were seeded on a 96-well plate followed by being incubated at 37°C for 6 h. The LDH solution (Mlbio, Shanghai, China) was subsequently added into each well, and cells were incubated for 1 h at room temperature. Finally, the absorbance at 492 nm was measured using a microplate reader (Mindray, Shenzhen, China) for the calculation of LDH release.
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