The bacterial species used were Proteus mirabilis (ATCC 29906), Escherichia fergusonii (ATCC 35469), Escherichia coli HB101 (Caenorhabditis Genetics Center), Pseudomonas fluorescens (ATCC 13525), Micrococcus luteus (ATCC 4698), Staphylococcus hominis (ATCC 27844), Rhizobium leguminosarum (ATCC 14479), Raoultella ornithinolytica JUb54 and Raoultella sp. JUb38 (obtained from Samuel lab, Baylor College of Medicine), and Bacteroides thetaiotaomicron (ATCC 29148, obtained from Hsiao lab, UCLA). E. coli, E. fergusonii, P. mirabilis, P. fluorescens, JUb38, and JUb54 were maintained on Luria broth (LB) plates, grown in LB, and diluted in LB. S. hominis and M. luteus were maintained on tryptic soy plates, grown in tryptic soy media, and diluted in tryptic soy media. R. leguminosarum was maintained on Rhizobium X plates, grown in Rhizobium X media, and diluted in Rhizobium X media. B. thetaiotaomicron was cultured anaerobically in brain-heart infusion supplemented media (BHIS) as previously described [70 (link)], and diluted in BHIS. Growth temperatures were 37 °C for all strains except for the Raoultella strains, P. fluorescens, and R. leguminosarum (28 °C); and M. luteus (30 °C). Each species was diluted to 1 × 108–1 × 109 CFU/mL as measured by a Thermo Scientific™ NanoDrop™ One spectrophotometer before use.
Bacteroides thetaiotaomicron
Manufactured by American Type Culture Collection
Sourced in United States
Bacteroides thetaiotaomicron is a type of anaerobic bacteria that can be obtained from the American Type Culture Collection (ATCC). It is a Gram-negative, non-spore-forming, and rod-shaped bacterium.
Automatically generated - may contain errors
Lab products found in correlation
Proteus mirabilis, by American Type Culture Collection (1 mentions)
Micrococcus luteus, by American Type Culture Collection (1 mentions)
Nanodrop one spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Staphylococcus hominis, by American Type Culture Collection (1 mentions)
Pseudomonas fluorescens, by American Type Culture Collection (1 mentions)
Bacillus subtilis, by American Type Culture Collection (1 mentions)
Parabacteroides distasonis, by American Type Culture Collection (1 mentions)
Prevotella nigrescens, by American Type Culture Collection (1 mentions)
Clostridium sporogenes, by American Type Culture Collection (1 mentions)
2 protocols using bacteroides thetaiotaomicron
1
Diverse Bacterial Species Cultivation
2
Clostridium Strains Cultivation and Maintenance
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Clostridium difficile strain 630 (ATCC® BAA-1382-FZTM), Clostridium difficile strain 4118 (ATCC® BAA-1870TM), Clostridium sporogenes (ATCC® 15579TM), Clostridium clostridioforme (ATCC® 25537TM), Bacteroides thetaiotaomicron (ATCC® 29148TM), Parabacteroides distasonis (ATCC® 8503TM) Prevotella nigrescens (ATCC® 33563TM), and Bacillus subtilis (ATCC® 6051TM) were purchased from ATCC (Manassas, VA, USA). Pseudomonas aeruginosa PAO1 was provided by Dr. Kendra Rumbaugh. Bacteroides fragilis strain CL05T00C42, Bifidobacterium longum strain 44B, Bifidobacterium breve strain EX336960VC19, Lactobacillus reuteri strain CF48-3A, Lactobacillus johnsonii strain 135-1-CHN, and all other C. difficile isolates described in this study were from BEI Resources, NIAID, NIH.
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