Bcar1

Manufactured by Abcam

BCAR1 is a protein that plays a role in cell adhesion and migration. It is expressed in various cell types and tissues. This product can be used for research purposes related to the study of BCAR1 and its functions.

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Lab products found in correlation

2 protocols using bcar1

Chromatin Immunoprecipitation and Antibody Validation

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The following antibodies were used: CDK7 (Cell Signaling Technology; 2916); RNAPII CTD S2 (Bethyl Laboratories; A300–654A), RNAPII CTD S5 (Bethyl Laboratories; A300–655A), RNAPII CTD S7 (Cell Signaling Technology; 13780), RNAPII (Santa Cruz Biotechnology; sc-899), BCAR1 (Abcam; ab80016), ETS2 (GeneTex; GTX104527 and Santa Cruz Biotechnology; sc-365666X), F3 (Novus Biologicals; TF9–10H10), LDLR (Abcam; ab52818), TBC1D2 (Novus Biologicals; NBP1–87335), GAPDH (Abcam; ab46540), anti-rabbit IgG (Cell Signaling Technology; 7074), H3K27ac (Abcam; ab4729), H3K4me1 (Abcam; ab8895), H3K4me3 (Abcam; ab8580), and RNA Pol II (Santa Cruz Biotechnology; sc-899 X).

Yuan J., Jiang Y.Y., Mayakonda A., Huang M., Ding L.W., Lin H., Yu F., Lu Y., Loh T.K., Chow M., Savage S., Tyner J.W., Lin D.C, & Koeffler H.P. (2017). Super-Enhancers Promote Transcriptional Dysregulation in Nasopharyngeal Carcinoma. Cancer research, 77(23), 6614-6626.

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Protein Expression Analysis by Western Blotting

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Western blotting was performed according to a published protocol [24 (link)]. In brief, proteins were extracted in RIPA lysis buffer supplemented with phosphatase and protease inhibitors (CWBIO, Shanghai, China), and protein concentration was quantified with BCA Protein Assay (Thermo Scientific, Waltham, MA, USA). The proteins were separated by 10% SDS-PAGE and then transferred onto polyvinyl difluoride membranes (EMD Millipore, Billerica, MA, USA), followed by incubation with primary antibodies overnight. Primary antibodies used were as follows: α-tubulin, β-actin, ASF1B, BCAR1, Slug, CDK2/4, CCND1, P27 (CST), Snail, Slug, E-cadherin, and Vimentin (Abcam).

Wu L, & Jie B. (2021). Protumor Effects of Histone H3–H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses. Computational and Mathematical Methods in Medicine, 2021, 5005459.

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